High-throughput micro-solid phase extraction on 96-well plate using dodecyl methacrylate-ethylen glycol dimethacrylate monolithic copolymer

A novel high-throughput device based on 96-micro-solid phase extraction (96-mu-SPE) system was constructed for multiresidue determination of nine pesticides in aquatic samples. The extraction procedure was performed on a commercially available 96-well plate system. The extraction module consisted of 96 pieces of 1 cm x 3 cm of cylindrically shaped stainless steel meshes. The prepared meshes were fixed in a home-made polytetrafluoroethylene-based constructed ninety-six holes block for possible simultaneous immersion of meshes into the center of individual wells. Dodecyl methacrylate and ethylene glycol dimethacrylate was copolymerized as a monolithic polymer and placed in the cylindrically shaped stainless steel meshes as extracting medium. A volume of 1 mL of the aquatic sample was transferred into the 96-well plate and the 96-mu-SPE device was applied for the extraction of the selected pesticides. Subsequently, the extracted analytes were analyzed by gas chromatography-mass spectrometry. Influential parameters such as polymer synthesis conditions, sorbent-to-sorbent reproducibility, ionic strength and extraction time were optimized. Intra and inter-sorbent reproducibility on 96-mu-SPE device were evaluated and results revealed that extraction yields are rather similar. Limits of detection were below 4 mu g L-1 and the coefficient of determination was satisfactory (r(2) > 0.99) for all the studied analytes. The developed method was successfully applied to the extraction and determination of the selected pesticides in surface water samples. (c) 2013 Elsevier B.V. All rights reserved.