Validated HPLC-Fl Method for the Analysis of S-Adenosylmethionine and S-Adenosylhomocysteine Biomarkers in Human Blood

被引：13
作者：
Albu, Camelia [1 ]
Litescu, Simona Carmen [1 ]
Radu, Gabriel Lucian [2 ]
Aboul-Enein, Hassan Y. [3 ]
机构：
[1] Natl Inst Biol Sci, Ctr Bioanal, Bucharest, Romania
[2] Univ Politehn Bucuresti, Fac Appl Chem & Mat Sci, Bucharest, Romania
[3] Natl Res Ctr, Pharmaceut & Med Chem Dept, Pharmaceut & Drug Ind Res Div, Cairo 12311, Egypt
来源：
JOURNAL OF FLUORESCENCE | 2013年 / 23卷 / 03期
关键词：
S-adenosylmethionine; S-adenosylhomocysteine; HPLC; Validation; Human blood; Biomarkers; TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; WHOLE-BLOOD; DISEASE; PLASMA; HOMOCYSTEINE; QUANTIFICATION; SAMPLES; SERUM;
D O I：
10.1007/s10895-013-1173-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The natural methyl donor group, S-adenosylmethionine and its product, S-adenosylhomocysteine play an important role in many biochemical reactions involving transmethylation reactions. These compounds can be used as biomarkers in incipient diagnosis of various pathological disorders therefore the validation of a suitable method to routinely analysis of these compounds is very important. In this paper, a high performance liquid chromatrography method for S-adenosylmethionine and S-adenosylhomocysteine measurement as fluorescent 1,N (6)-ethanoderivatives from biological samples was validated in terms of selectivity, linearity range of the response (R > 0.9993), detection limit (9 x 10(-9) and 4.4 x 10(-9) molL(-1)), the limit of quantitation (9.7 x 10(-9) and 5.7 x 10(-9) mol L-1), precision, trueness and robustness. The method for quantification simultaneous of these compounds is rapid, sensitive and precise and appropriate for clinical analysis.
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收藏
页码：381 / 386
页数：6
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