Evaluation of a digoxigenin-labelled probe for detection of Aeromonas spp.

被引：7
作者：
Khushiramani, R. [1 ]
Girisha, S. K. [1 ]
Karunasagar, I. [1 ]
Karunasagar, I. [1 ]
机构：
[1] Karnataka Vet Anim & Fisheries Sci Univ, Coll Fisheries, Dept Fishery Microbiol, Mangalore, India
来源：
LETTERS IN APPLIED MICROBIOLOGY | 2009年 / 48卷 / 03期
关键词：
Aeromonas; detection; digoxigenin; gram-negative bacterium; probe; OUTER-MEMBRANE PROTEIN; HYDROPHILA; GENES; OMPA; FISH;
D O I：
10.1111/j.1472-765X.2008.02491.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An outer membrane protein-based Digoxigenin (DIG)-labelled DNA probe was developed for the specific detection of Aeromonas sp. from food/environmental/clinical samples. Dot blot reaction answered for all the Aeromonas isolates and was negative for Escherichia coli, Pseudomonas sp., Klebsiella sp., Vibrio parahaemolyticus, V. harveyi, V. alginolyticus, V. vulnificus. Edwardsiella tarda and Staphylococcus sp. As this protein is highly conserved in various Aeromonas species, the probe has the potential for use as a rapid and reliable diagnostic tool.
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页码：383 / 385
页数：3
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