Type 1 ribosome-inactivating proteins are the most abundant proteins in iris (Iris hollandica var Professor Blaauw) bulbs: Characterization and molecular cloning

被引：33
作者：
VanDamme, EJM
Barre, A
Barbieri, L
Valbonesi, P
Rouge, P
VanLeuven, F
Stirpe, F
Peumans, WJ
机构：
[1] UNIV TOULOUSE 3, INST PHARMACOL & BIOL STRUCT, CNRS, UPR 9062, F-31062 TOULOUSE, FRANCE
[2] UNIV BOLOGNA, DIPARTIMENTO PATOL SPERIMENTALE, I-40126 BOLOGNA, ITALY
[3] CATHOLIC UNIV LEUVEN, CTR HUMAN GENET, B-3001 LOUVAIN, BELGIUM
来源：
BIOCHEMICAL JOURNAL | 1997年 / 324卷
关键词：
D O I：
10.1042/bj3240963
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The most abundant protein of Iris bulbs has been identified as a type 1 ribosome-inactivating protein (RIP). Analysis of the purified proteins and molecular cloning of the corresponding cDNAs demonstrated that this type 1 RIP is a mixture of three isoforms that exhibit a high degree of sequence identity and have similar, though not identical, ribosome-inactivating and polynucleotide :adenosine glycosidase activities. The accumulation of large quantities of type 1 RIP in a vegetative storage organ suggests that this presumed defence-related protein also plays a role in the nitrogen-storage metabolism of the bulb.
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页码：963 / 970
页数：8
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