Classic "broken cell" techniques and newer live cell methods for cell cycle assessment

被引:16
|
作者
Henderson, Lindsay [1 ]
Bortone, Dante S. [1 ]
Lim, Curtis [1 ]
Zambon, Alexander C. [2 ,3 ]
机构
[1] Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA
[3] Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 2013年 / 304卷 / 10期
基金
美国国家卫生研究院;
关键词
cell cycle pathway; live cells; sensors; analysis methods; review; FLUORESCENT PROTEIN; DNA-REPLICATION; GENE-EXPRESSION; IN-VIVO; PHOSPHORYLATION SITES; NEGATIVE REGULATORS; SPINDLE FORMATION; PROLIFERATION; APOPTOSIS; DYNAMICS;
D O I
10.1152/ajpcell.00006.2013
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Many common, important diseases are either caused or exacerbated by hyperactivation (e.g., cancer) or inactivation (e.g., heart failure) of the cell division cycle. A better understanding of the cell cycle is critical for interpreting numerous types of physiological changes in cells. Moreover, new insights into how to control it will facilitate new therapeutics for a variety of diseases and new avenues in regenerative medicine. The progression of cells through the four main phases of their division cycle [G(0)/G(1), S (DNA synthesis), G(2), and M (mitosis)] is a highly conserved process orchestrated by several pathways (e.g., transcription, phosphorylation, nuclear import/export, and protein ubiquitination) that coordinate a core cell cycle pathway. This core pathway can also receive inputs that are cell type and cell niche dependent. "Broken cell" methods (e.g., use of labeled nucleotide analogs) to assess for cell cycle activity have revealed important insights regarding the cell cycle but lack the ability to assess living cells in real time (longitudinal studies) and with single-cell resolution. Moreover, such methods often require cell synchronization, which can perturb the pathway under study. Live cell cycle sensors can be used at single-cell resolution in living cells, intact tissue, and whole animals. Use of these more recently available sensors has the potential to reveal physiologically relevant insights regarding the normal and perturbed cell division cycle.
引用
收藏
页码:C927 / C938
页数:12
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