Microcomposition of Human Urinary Calculi Using Advanced Imaging Techniques

被引:26
作者
Blaschko, Sarah D. [1 ]
Miller, Joe [1 ]
Chi, Thomas [1 ]
Flechner, Lawrence [1 ]
Fakra, Sirine [2 ]
Kahn, Arnold [3 ]
Kapahi, Pankaj [3 ]
Stoller, Marshall L. [1 ]
机构
[1] Univ Calif San Francisco, Dept Urol, San Francisco, CA 94143 USA
[2] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Adv Light Source, Berkeley, CA 94720 USA
[3] Buck Inst Res Aging, Novato, CA USA
基金
美国国家卫生研究院; 美国能源部;
关键词
kidney; diagnostic imaging; nephrolithiasis; urolithiasis; radiography; KIDNEY-STONES; CALCIUM-OXALATE; MICROANALYSIS; SPECTROSCOPY; ZN;
D O I
10.1016/j.juro.2012.09.098
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Purpose: Common methods of commercial urolithiasis analysis, such as light microscopy and Fourier transform infrared spectroscopy, provide limited or no information on the molecular composition of stones, which is vital when studying early stone pathogenesis. We used synchrotron radiation based microfocused x-ray fluorescence, x-ray absorption and x-ray diffraction advanced imaging techniques to identify and map the elemental composition, including trace elements, of urinary calculi on a mu m (0.0001 cm) scale. Materials and Methods: Human stone samples were obtained during serial percutaneous nephrolithotomy and ureteroscopy procedures. A portion of each sample was sent for commercial stone analysis and a portion was retained for synchrotron radiation based advanced imaging analysis. Results: Synchrotron radiation based methods of stone analysis correctly identified stone composition and provided additional molecular detail on elemental components and spatial distribution in uroliths. Resolution was on the order of a few mu m. Conclusions: Knowledge of all elements present in lithogenesis at this detail allows for better understanding of early stone formation events, which may provide additional insight to prevent and treat stone formation.
引用
收藏
页码:726 / 734
页数:9
相关论文
共 16 条
[1]   Revisiting the localisation of Zn2+ cations sorbed on pathological apatite calcifications made through X-ray absorption spectroscopy [J].
Bazin, Dominique ;
Carpentier, Xavier ;
Brocheriou, Isabelle ;
Dorfmuller, Peter ;
Aubert, Stephane ;
Chappard, Christine ;
Thiaudiere, Dominique ;
Reguer, Solenn ;
Waychunas, Glenn ;
Jungers, Paul ;
Daudon, Michel .
BIOCHIMIE, 2009, 91 (10) :1294-1300
[2]   CYSTINE CALCULI ROUGH AND SMOOTH - A NEW CLINICAL DISTINCTION [J].
BHATTA, KM ;
PRIEN, EL ;
DRETLER, SP .
JOURNAL OF UROLOGY, 1989, 142 (04) :937-940
[3]   High Zn content of Randall's plaque: A μ-X-ray fluorescence investigation [J].
Carpentier, Xavier ;
Bazin, Dominique ;
Combes, Christelle ;
Mazouyes, Aurelie ;
Rouziere, Stephan ;
Albouy, Pierre Antoine ;
Foy, Eddy ;
Daudon, Michel .
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY, 2011, 25 (03) :160-165
[4]  
Griffiths P. R., 2006, Fourier Transform Infrared Spectrometry
[5]  
HESSE A, 1977, EUR UROL, V3, P359
[6]   Quality control in urinary stone analysis: results of 44 ring trials (1980-2001) [J].
Hesse, A ;
Kruse, R ;
Geilenkeuser, WJ ;
Schmidt, M .
CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2005, 43 (03) :298-303
[7]   IDENTIFICATION OF URINARY STONE AND SEDIMENT CRYSTALS BY SCANNING ELECTRON-MICROSCOPY AND X-RAY-MICROANALYSIS [J].
KHAN, SR ;
HACKETT, RL .
JOURNAL OF UROLOGY, 1986, 135 (04) :818-825
[8]   Calcium phosphate calcium oxalate crystal association in urinary stones: Implications for heterogeneous nucleation of calcium oxalate [J].
Khan, SR .
JOURNAL OF UROLOGY, 1997, 157 (01) :376-383
[9]   Analysis of mixed stones is prone to error: a study with US laboratories using micro CT for verification of sample content [J].
Krambeck, Amy E. ;
Lingeman, James E. ;
McAteer, James A. ;
Williams, James C., Jr. .
UROLOGICAL RESEARCH, 2010, 38 (06) :469-475
[10]  
Mandel I, 2007, CURR CLIN UROL, P69, DOI 10.1007/978-1-59259-972-1_5