Stathmin Regulates Microtubule Dynamics and Microtubule Organizing Center Polarization in Activated T Cells

被引：41

作者：

Filbert, Erin L. ^{[1
]}

Le Borgne, Marie ^{[1
,2
]}

Lin, Joseph ^{[1
]}

Heuser, John E. ^{[3
]}

Shaw, Andrey S. ^{[1
,2
]}

机构：

[1] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA

[2] Washington Univ, Sch Med, Howard Hughes Med Inst, St Louis, MO 63110 USA

[3] Washington Univ, Sch Med, Dept Cell Biol, St Louis, MO 63110 USA

来源：

JOURNAL OF IMMUNOLOGY | 2012年 / 188卷 / 11期

基金：

美国国家卫生研究院;

关键词：

IMMUNOLOGICAL SYNAPSE; PROTEIN-KINASE; CYTOSKELETAL POLARIZATION; CYTOSOLIC TARGET; ANTIGEN RECEPTOR; GOLGI-APPARATUS; PHOSPHORYLATION; PHOSPHOPROTEIN; LYMPHOCYTES; PATHWAYS;

D O I：

10.4049/jimmunol.1200242

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Polarization of T cells involves reorientation of the microtubule organizing center (MTOC). Because activated ERK is localized at the immunological synapse, we investigated its role by showing that ERK activation is important for MTOC polarization. Suspecting that ERK phosphorylates a regulator of microtubules, we next focused on stathmin, a known ERK substrate. Our work indicates that during T cell activation, ERK is recruited to the synapse, allowing it to phosphorylate stathmin molecules near the immunological synapse. Supporting an important role of stathmin phosphorylation in T cell activation, we showed that T cell activation results in increased microtubule growth rate dependent on the presence of stathmin. The significance of this finding was demonstrated by results showing that CTLs from stathmin(-/-) mice displayed defective MTOC polarization and defective target cell cytolysis. These data implicate stathmin as a regulator of the microtubule network during T cell activation. The Journal of Immunology, 2012, 188: 5421-5427.

引用

页码：5421 / 5427

页数：7

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