Time-resolved step-scan Fourier transform infrared investigation of heme-copper oxidases:: implications for O2 input and H2O/H+ output channels

被引：24
作者：
Koutsoupakis, C [1 ]
Pinakoulaki, E [1 ]
Stavrakis, S [1 ]
Daskalakis, V [1 ]
Varotsis, C [1 ]
机构：
[1] Univ Crete, Dept Chem, Iraklion 71409, Crete, Greece
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 2004年 / 1655卷 / 1-3期
关键词：
D O I：
10.1016/j.bbabio.2003.06.004
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have applied FTIR and time-resolved step-scan Fourier transform infrared (TRS2-FTIR) spectroscopy to investigate the dynamics of the heme-Cu-B binuclear center and the protein dynamics of mammalian aa(3), Pseudomonas stutzeri cbb(3), and caa(3) and ba(3) from Thermus themrophilus cytochrome oxidases. The implications of these results with respect to (1) the molecular motions that are general to the photodynamics of the binuclear center in heme-copper oxidases, and (2) the proton pathways located in the ring A propionate of heme a(3)-Asp372-H2O site that is conserved anion, all structurally known oxidases are discussed. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：347 / 352
页数：6
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