Alkaline polyethylene glycol-based method for direct PCR from bacteria, eukaryotic tissue samples, and whole blood

被引：136
作者：
Chomczynski, P [1 ]
Rymaszewski, M [1 ]
机构：
[1] Mol Res Ctr, Cincinnati, OH 45212 USA
来源：
BIOTECHNIQUES | 2006年 / 40卷 / 04期
关键词：
D O I：
10.2144/000112149
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
[No abstract available]
引用
收藏
页码：454 / +
页数：3
相关论文
共 8 条
  • [1] AGUDO LDC, 1995, BIOTECHNIQUES, V18, P766
  • [2] Effect of polyethylene glycol on the liquid-liquid phase transition in aqueous protein solutions
    Annunziata, O
    Asherie, N
    Lomakin, A
    Pande, J
    Ogun, O
    Benedek, GB
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (22) : 14165 - 14170
  • [3] Klintschar M, 2000, J FORENSIC SCI, V45, P669
  • [4] Modulation of lipid phase behavior by kosmotropic and chaotropic solutes - Experiment and thermodynamic theory
    Koynova, R
    Brankov, J
    Tenchov, B
    [J]. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 1997, 25 (04): : 261 - 274
  • [5] Rapid, simple alkaline extraction of human genomic DNA from whole blood, buccal epithelial cells, semen and forensic stains for PCR
    Rudbeck, L
    Dissing, J
    [J]. BIOTECHNIQUES, 1998, 25 (04) : 588 - +
  • [6] DNA extraction from archival formalin-fixed, paraffin-embedded tissues: heat-induced retrieval in alkaline solution
    Shi, SR
    Datar, R
    Liu, C
    Wu, L
    Zhang, Z
    Cote, RJ
    Taylor, CR
    [J]. HISTOCHEMISTRY AND CELL BIOLOGY, 2004, 122 (03) : 211 - 218
  • [7] Sofer G, 2003, BIOPHARM INT, V16, pS37
  • [8] von Ahsen N, 2000, CLIN CHEM, V46, P156
1