Exosomes released in vitro from Epstein-Barr virus (EBV)-infected cells contain EBV-encoded latent phase mRNAs

被引：79
作者：
Canitano, Andrea [1 ]
Venturi, Giulietta [1 ]
Borghi, Martina [1 ]
Ammendolia, Maria Grazia [2 ]
Fais, Stefano [1 ]
机构：
[1] Ist Super Sanita, Dept Therapeut Res & Med Evaluat, I-00161 Rome, Italy
[2] Ist Super Sanita, Dept Technol & Hlth, I-00161 Rome, Italy
来源：
CANCER LETTERS | 2013年 / 337卷 / 02期
关键词：
EBV; Exosomes; mRNA; miRNA; Latent infection; NASOPHARYNGEAL CARCINOMA-CELLS; MEMBRANE PROTEIN-1; MICRORNAS; INFECTION; MIRNAS; LMP1; TRANSFORMATION; MICROVESICLES; LOCALIZATION; EXPRESSION;
D O I：
10.1016/j.canlet.2013.05.012
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
EBV is a human herpesvirus associated with a number of malignancies. Both lymphoblastoid cell lines (LCLs), and EBV-infected nasopharyngeal carcinoma (NPC) cells have been demonstrated to release exosomes containing the EBV-encoded latent membrane protein 1 (LMP1), and mature micro-RNAs (EBV-miRNAs). Here we analyze the EBV protein and nucleic acid content of exosomes from different EBV-infected cells (LCL, 721 and Daudi) and we show for the first time that exosomes released from LCLs and 721 also contain EBV-encoded latent phase mRNAs. This confirms and strengthens exosomes pathogenetic potential, and might provide insights for development of novel diagnostic and therapeutic strategies. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：193 / 199
页数：7
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