Gene expression profiling in single cells within tissue

被引：41

作者：

Capodieci, P

Donovan, M

Buchinsky, H

Jeffers, Y

Cordon-Cardo, C

Gerald, W

Edelson, J

Shenoy, SM

Singer, RH

机构：

[1] Aureon Labs, Yonkers, NY 10701 USA

[2] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA

[3] Albert Einstein Coll Med, Bronx, NY 10461 USA

来源：

NATURE METHODS | 2005年 / 2卷 / 09期

关键词：

D O I：

10.1038/NMETH786

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.

引用

页码：663 / 665

页数：3

共 15 条

[11] Genomics, gene expression and DNA arrays
Lockhart, DJ
Winzeler, EA
[J]. NATURE, 2000, 405 (6788) : 827 - 836
[12] MicroRNA expression profiles classify human cancers
Lu, J
Getz, G
Miska, EA
Alvarez-Saavedra, E
Lamb, J
Peck, D
Sweet-Cordero, A
Ebet, BL
Mak, RH
Ferrando, AA
Downing, JR
Jacks, T
Horvitz, HR
Golub, TR
[J]. NATURE, 2005, 435 (7043) : 834 - 838
[13] A multigene assay to predict recurrence of tamoxifen-treated, node-negative breast cancer
Paik, S
Shak, S
Tang, G
Kim, C
Baker, J
Cronin, M
Baehner, FL
Walker, MG
Watson, D
Park, T
Hiller, W
Fisher, ER
Wickerham, DL
Bryant, J
Wolmark, N
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 2004, 351 (27) : 2817 - 2826
[14] A gene-expression signature as a predictor of survival in breast cancer.
van de Vijver, MJ
He, YD
van 't Veer, LJ
Dai, H
Hart, AAM
Voskuil, DW
Schreiber, GJ
Peterse, JL
Roberts, C
Marton, MJ
Parrish, M
Atsma, D
Witteveen, A
Glas, A
Delahaye, L
van der Velde, T
Bartelink, H
Rodenhuis, S
Rutgers, ET
Friend, SH
Bernards, R
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 2002, 347 (25) : 1999 - 2009
[15] Wilson AJ, 2002, CANCER RES, V62, P6006

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