Gene expression profiling in single cells within tissue

被引：41
作者：
Capodieci, P
Donovan, M
Buchinsky, H
Jeffers, Y
Cordon-Cardo, C
Gerald, W
Edelson, J
Shenoy, SM
Singer, RH
机构：
[1] Aureon Labs, Yonkers, NY 10701 USA
[2] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA
[3] Albert Einstein Coll Med, Bronx, NY 10461 USA
来源：
NATURE METHODS | 2005年 / 2卷 / 09期
关键词：
D O I：
10.1038/NMETH786
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.
引用
收藏
页码：663 / 665
页数：3
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