Evaluation of Storage Tubes for Combined Analysis of Circulating Nucleic Acids in Liquid Biopsies

被引:47
作者
Gahlawat, Aoife Ward [1 ]
Lenhardt, Judith [1 ]
Witte, Tania [1 ]
Keitel, Denise [1 ]
Kaufhold, Anna [2 ]
Maass, Kendra K. [2 ]
Pajtler, Kristian W. [2 ,3 ]
Sohn, Christof [1 ]
Schott, Sarah [1 ]
机构
[1] Univ Hosp Heidelberg, Dept Gynecol & Obstet, D-69120 Heidelberg, Germany
[2] German Canc Res Ctr, Div Pediat Neurooncol, D-69120 Heidelberg, Germany
[3] Univ Hosp Heidelberg, Dept Pediat Oncol Hematol & Immunol, D-69120 Heidelberg, Germany
关键词
circulating miRNA; cell-free DNA; blood storage; blood collection tubes; BLOOD COLLECTION TUBES; CELL-FREE DNA; TUMOR-CELLS; MICRORNAS; PLASMA; MARKERS; RNA; GENES;
D O I
10.3390/ijms20030704
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the last decade, circulating nucleic acids such as microRNAs (miRNAs) and cell-free DNA (cfDNA) have become increasingly important in serving as potential novel biomarkers for a variety of human diseases. If cell-free nucleic acids are to become routinely used in diagnostics, the difference in plasma miRNA and cfDNA levels between healthy and diseased subjects must exceed pre-analytical and analytical variability. Until now, few studies have addressed the time limitations of pre-processing or explored the potential use of long-term blood storage tubes, which might need to be implemented in real-life diagnostics. In this study, we analyzed the stability of four breast cancer-associated miRNAs and two cancer-associated genes under various storage conditions, to test their limitations for potential application in clinical diagnostics. In two consecutive experiments, we tested the limits of conventional EDTA tubes, as well as long-term storage blood collection tubes (BCTs) from four different manufacturers. We found that circulating miRNAs are relatively stable when stored in EDTA monovettes for up to 12 h before processing. When stored in BCTs, circulating miRNAs and cfDNA are stable for up to 7 days, depending on the manufacturer. Norgen tubes were superior for cfDNA yield, while Streck tubes performed the worst in our study with hemolysis induction. In conclusion, plasma prepared from whole blood is suitable for the quantification of both cf-miRNAs and cfDNA simultaneously.
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页数:10
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共 27 条
[1]   Comparison of Blood Collection Tubes from Three Different Manufacturers for the Collection of Cell-Free DNA for Liquid Biopsy Mutation Testing [J].
Alidousty, Christina ;
Brandes, Danielle ;
Heydt, Carina ;
Wagener, Svenja ;
Wittersheim, Maike ;
Schaefer, Stephan C. ;
Holz, Barbara ;
Merkelbach-Bruse, Sabine ;
Buettner, Reinhard ;
Fassunke, Jana ;
Schultheis, Anne M. .
JOURNAL OF MOLECULAR DIAGNOSTICS, 2017, 19 (05) :801-804
[2]   MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].
Bartel, David P. .
CELL, 2007, 131 (04) :11-29
[3]   Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia [J].
Calin, GA ;
Dumitru, CD ;
Shimizu, M ;
Bichi, R ;
Zupo, S ;
Noch, E ;
Aldler, H ;
Rattan, S ;
Keating, M ;
Rai, K ;
Rassenti, L ;
Kipps, T ;
Negrini, M ;
Bullrich, F ;
Croce, CM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (24) :15524-15529
[4]   Detection and localization of surgically resectable cancers with a multi-analyte blood test [J].
Cohen, Joshua D. ;
Li, Lu ;
Wang, Yuxuan ;
Thoburn, Christopher ;
Afsari, Bahman ;
Danilova, Ludmila ;
Douville, Christopher ;
Javed, Ammar A. ;
Wong, Fay ;
Mattox, Austin ;
Hruban, Ralph. H. ;
Wolfgang, Christopher L. ;
Goggins, Michael G. ;
Dal Molin, Marco ;
Wang, Tian-Li ;
Roden, Richard ;
Klein, Alison P. ;
Ptak, Janine ;
Dobbyn, Lisa ;
Schaefer, Joy ;
Silliman, Natalie ;
Popoli, Maria ;
Vogelstein, Joshua T. ;
Browne, James D. ;
Schoen, Robert E. ;
Brand, Randall E. ;
Tie, Jeanne ;
Gibbs, Peter ;
Wong, Hui-Li ;
Mansfield, Aaron S. ;
Jen, Jin ;
Hanash, Samir M. ;
Falconi, Massimo ;
Allen, Peter J. ;
Zhou, Shibin ;
Bettegowda, Chetan ;
Diaz, Luis A., Jr. ;
Tomasetti, Cristian ;
Kinzler, Kenneth W. ;
Vogelstein, Bert ;
Lennon, Anne Marie ;
Papadopoulos, Nickolas .
SCIENCE, 2018, 359 (6378) :926-+
[5]   Plasma MicroRNA Panel for Minimally Invasive Detection of Breast Cancer [J].
Cuk, Katarina ;
Zucknick, Manuela ;
Madhavan, Dharanija ;
Schott, Sarah ;
Golatta, Michael ;
Heil, Joerg ;
Marme, Frederik ;
Turchinovich, Andrey ;
Sinn, Peter ;
Sohn, Christof ;
Junkermann, Hans ;
Schneeweiss, Andreas ;
Burwinkel, Barbara .
PLOS ONE, 2013, 8 (10)
[6]   Circulating microRNAs in plasma as early detection markers for breast cancer [J].
Cuk, Katarina ;
Zucknick, Manuela ;
Heil, Joerg ;
Madhavan, Dharanija ;
Schott, Sarah ;
Turchinovich, Andrey ;
Arlt, Dorit ;
Rath, Michelle ;
Sohn, Christof ;
Benner, Axel ;
Junkermann, Hans ;
Schneeweiss, Andreas ;
Burwinkel, Barbara .
INTERNATIONAL JOURNAL OF CANCER, 2013, 132 (07) :1602-1612
[7]   Stabilization of Cellular RNA in Blood During Storage at Room Temperature: A Comparison of Cell-Free RNA BCT® with K3EDTA Tubes [J].
Das, Kausik ;
Norton, Sheila E. ;
Alt, Jodi R. ;
Krzyzanowski, Gary D. ;
Williams, Thomas L. ;
Fernando, M. Rohan .
MOLECULAR DIAGNOSIS & THERAPY, 2014, 18 (06) :647-653
[8]   Circulating tumour cells and cell-free DNA as tools for managing breast cancer [J].
De Mattos-Arruda, Leticia ;
Cortes, Javier ;
Santarpia, Libero ;
Vivancos, Ana ;
Tabernero, Josep ;
Reis-Filho, Jorge S. ;
Seoane, Joan .
NATURE REVIEWS CLINICAL ONCOLOGY, 2013, 10 (07) :377-389
[9]   Robust MicroRNA Stability in Degraded RNA Preparations from Human Tissue and Cell Samples [J].
Jung, Monika ;
Schaefer, Annika ;
Steiner, Isabel ;
Kempkensteffen, Carsten ;
Stephan, Carsten ;
Erbersdobler, Andreas ;
Jung, Klaus .
CLINICAL CHEMISTRY, 2010, 56 (06) :998-1006
[10]   Sources to variability in circulating human miRNA signatures [J].
Keller, Andreas ;
Rounge, Trine ;
Backes, Christina ;
Ludwig, Nicole ;
Gislefoss, Randi ;
Leidinger, Petra ;
langseth, HilDe ;
Meese, Eckart .
RNA BIOLOGY, 2017, 14 (12) :1791-1798