Individual Indentification and Parentage Verification of Thoroughbred Horses and the Korean Native Horses Based on Microsatellite Loci in Korea

This study was performed for individual identification and parentage verification of Thoroughbred Horse (TBH) and the Korean Native Horse (KNH). A total number of 1,308 (84 KNH and 1,224 TBH samples including 1,005 foals for parentage testing) were genotyped. Genomic DNAs were extracted from whole blood of hair roots and genotyped by using 14 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS3, HMS6, HMS7, HTG4, HTG10, LEX3 and VHL20) in TBH and 17 microsatellite markers including HMS2, HTG6, HTG7 in the KNH. This method consisted of multiplexing PCR procedure and showed reasonable amplification of all PCR products. Genotyping were determined with an ABI 3100 genetic analyzer. All the loci analyzed in 17 microsatellite markers showed a polymorphic patterns. The number of alleles per locus varied from 3-9 with a mean value of 6.36 and 5-10 (mean 7.35) in TBH and KNH, respectively. The expected heterozygosity was ranged from 0.539-0.827 (mean 0.700), from 0.387-0.841 (mean 0.702) in TBH and KNH, respectively. The total exclusion probability of 14 microsatellite loci was 0.9998 in TBH in the KNH. Of the 14-17markers, ASB2, ASB17, ASB23, HMS7, HTG10 and LEX3 loci in TBH and AHT4, AHT5, CA425, HMS2, HMS3, HTG10 and LEX3 loci in the KNH have relatively high PIC value (>0.7). Of the 1005 foals, 1003 foals (99.80%) were qualified by compatibility according to the Mendelian fashion. These results indicated that the present microsatellites serve as useful tools for individual indentification and paternity testing of TBH and the KNH in Korea.