Identification, cloning, and expression of potential diagnostic markers for Q fever

被引：10
作者：
Chao, CC
Chen, HW
Li, X
Xu, WB
Hanson, B
Ching, WM
机构：
[1] USN, Med Res Ctr, RDD, IDD, Silver Spring, MD 20910 USA
[2] Uniformed Serv Univ Hlth Sci, Dept Prevent Med & Biometr, Bethesda, MD 20814 USA
[3] PanBio Inc, Columbia, MD USA
来源：
RICKETTSIOSES: FROM GENOME TO PROTEOME, PATHOBIOLOGY, AND RICKETTSIAE AS AN INTERNATIONAL THREAT | 2005年 / 1063卷
关键词：
Q fever; 2D gel electrophoresis; LC-MS-MS; protein antigens; biotin;
D O I：
10.1196/annals.1355.010
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The clinical diagnosis of Q fever is difficult. Whole cell antigens are currently used in several serological methods, but antigens are limited due to the hazardous nature of Coxiella burnetii cultivation. In this report, we described the method of detecting immunodominant antigens of C. burnetii by using proteomic techniques with patient sera, and cloning and expressing the selected antigens using a novel vector known for its ease of expression, purification, and downstream application.
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页码：76 / 78
页数：3
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