Sample digestion for determining chloramphenicol residues in carp serum and muscle

被引：4

作者：

Huang, Zhi-Yong ^{[1
]}

Yan, Qing-Pi ^{[2
]}

Zhang, Qiang ^{[1
]}

Peng, Ai-Hong ^{[1
]}

机构：

[1] Jimei Univ, Coll Bioengn, Xiamen 361021, Peoples R China

[2] Jimei Univ, Coll Fisheries, Xiamen 361021, Peoples R China

来源：

AQUACULTURE INTERNATIONAL | 2009年 / 17卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Cyprinus carpio L. (Carp); Chloramphenicol; Chloramphenicol glucuronide (CAP-G); Residue; TANDEM MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; GLUCURONIDE; OXYTETRACYCLINE; WATER; PHARMACEUTICALS; CONFIRMATION; AQUACULTURE; BIOSENSOR; DEPLETION;

D O I：

10.1007/s10499-008-9181-y

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Chloramphenicol (CAP) residues in carp (Cyprinus carpio L.) were investigated after intramuscular injection of a single-dose of 80 mg/kgbw. CAP concentrations in carp serum and muscle with and without addition of beta-glucuronidase were determined by high-performance liquid chromatography (HPLC) with UV detection. The results showed that CAP-glucuronide (CAP-G), an important conjugate of CAP in the metabolic process and potentially toxic to human health, was observed after the fish were injected with CAP. CAP concentrations in serum and muscle with added beta-glucuronidase were significantly (p < 0.05) higher than those without added enzyme. For example, within 24 h of injection, average CAP concentrations in serum and muscle with enzyme digestion were 30 and 48% more than those without the enzyme. In order to exactly determine the trace CAP content of fish, for evaluation of food safety, it is suggested that samples be hydrolyzed with enzyme (e.g., beta-glucuronidase).

引用

页码：69 / 76

页数：8

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