Enhanced contrast coherent anti-Stokes Raman scattering microscopy using annular phase masks

被引:8
作者
Beversluis, Michael R. [1 ]
Stranick, Stephan J. [1 ]
机构
[1] Natl Inst Stand & Technol, Gaithersburg, MD 20899 USA
关键词
coherent antiStokes Raman scattering; liquid crystal devices; optical microscopy; spatial light modulators;
D O I
10.1063/1.3046719
中图分类号
O59 [应用物理学];
学科分类号
摘要
We have experimentally demonstrated enhanced image contrast of sample fine structure in a coherent anti-Stokes Raman scattering (CARS) microscope using liquid crystal spatial light modulators as programmable Toraldo-style pupil phase masks. We demonstrated through modeling, implementation, and verification of the focal intensity and phase distributions of the excitation beams the trade-offs between improved image contrast and CARS signal levels.
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页数:3
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共 18 条
  • [11] Focus-engineered coherent anti-Stokes Raman scattering microscopy: a numerical investigation
    Krishnamachari, Vishnu Vardhan
    Potma, Eric Olaf
    [J]. JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2007, 24 (04) : 1138 - 1147
  • [12] Differential imaging in coherent anti-Stokes Raman scattering microscopy with Laguerre-Gaussian excitation beams
    Liu, Cheng
    Kim, Dug Young
    [J]. OPTICS EXPRESS, 2007, 15 (16): : 10123 - 10134
  • [13] Nonperturbative chemical imaging of organelle transport in living cells with coherent anti-stokes Raman scattering microscopy
    Nan, Xiaolin
    Potma, Eric O.
    Xie, X. Sunney
    [J]. BIOPHYSICAL JOURNAL, 2006, 91 (02) : 728 - 735
  • [14] Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stokes Raman scattering microscopy
    Nan, XL
    Cheng, JX
    Xie, XS
    [J]. JOURNAL OF LIPID RESEARCH, 2003, 44 (11) : 2202 - 2208
  • [15] Heterodyne coherent anti-Stokes Raman scattering (CARS) imaging
    Potma, EO
    Evans, CL
    Xie, XS
    [J]. OPTICS LETTERS, 2006, 31 (02) : 241 - 243
  • [16] ELECTROMAGNETIC DIFFRACTION IN OPTICAL SYSTEMS .2. STRUCTURE OF THE IMAGE FIELD IN AN APLANATIC SYSTEM
    RICHARDS, B
    WOLF, E
    [J]. PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL AND PHYSICAL SCIENCES, 1959, 253 (1274): : 358 - 379
  • [17] Volkmer A, 2002, BIOPHYS J, V82, p14A
  • [18] Adaptive optics for enhanced signal in CARS microscopy
    Wright, A. J.
    Poland, S. P.
    Girkin, J. M.
    Freudiger, C. W.
    Evans, C. L.
    Xie, X. S.
    [J]. OPTICS EXPRESS, 2007, 15 (26): : 18209 - 18219