Poly(ethylene glycol) hydrogel microstructures encapsulating living cells

被引：195
|
作者：
Koh, WG
Revzin, A
Pishko, MV [1 ]
机构：
[1] Penn State Univ, Dept Chem Engn, University Pk, PA 16802 USA
[2] Texas A&M Univ, Dept Chem Engn, College Stn, TX 77843 USA
来源：
LANGMUIR | 2002年 / 18卷 / 07期
关键词：
D O I：
10.1021/la0115740
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We present an easy and effective method for the encapsulation of cells inside PEG-based hydrogel microstructures fabricated using photolithography. High-density arrays of three-dimensional microstructures were created on substrates using this method. Mammalian cells were encapsulated in cylindrical hydrogel microstructures of 600 and 50 mum in diameter or in cubic hydrogel structures in microfluidic channels. Reducing lateral dimension of the individual hydrogel microstructure to 50 mum allowed us to isolate 1-3 cells per microstructure. Viability assays demonstrated that cells remained viable inside these hydrogels after encapsulation for up to 7 days.
引用
收藏
页码：2459 / 2462
页数：4
相关论文
共 50 条
  • [21] Synthesis and characterization of injectable, thermosensitive, and biocompatible acellular bone matrix/poly(ethylene glycol)-poly (ε-caprolactone)-poly(ethylene glycol) hydrogel composite
    Ni, Pei-Yan
    Fan, Min
    Qian, Zhi-Yong
    Luo, Jing-Cong
    Gong, Chang-Yang
    Fu, Shao-Zhi
    Shi, Shuai
    Luo, Feng
    Yang, Zhi-Ming
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2012, 100A (01) : 171 - 179
  • [22] Hybrid hierarchical patterns of gold nanoparticles and poly(ethylene glycol) microstructures
    Chen, Jingyu
    Arafeh, Manar
    Guiet, Amandine
    Felkel, Diana
    Loebus, Axel
    Kelleher, Susan M.
    Fischer, Anna
    Lensen, Marga C.
    JOURNAL OF MATERIALS CHEMISTRY C, 2013, 1 (46) : 7709 - 7715
  • [23] Degradative properties and cytocompatibility of a mixed-mode hydrogel containing oligo[poly(ethylene glycol) fumarate] and poly(ethylene glycol)dithiol
    Brink, Kelly S.
    Yang, Peter J.
    Temenoff, Johnna S.
    ACTA BIOMATERIALIA, 2009, 5 (02) : 570 - 579
  • [24] Safety of poly(ethylene glycol) and poly(ethylene glycol) derivatives
    Working, PK
    Newman, MS
    Johnson, J
    Cornacoff, JB
    POLY(ETHYLENE GLYCOL): CHEMISTRY AND BIOLOGICAL APPLICATIONS, 1997, 680 : 45 - 57
  • [25] Thermosensitive in situ hydrogel of paclitaxel conjugated poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone)
    Lin, Xiaona
    Deng, Liandong
    Xu, Yongshen
    Dong, Anjie
    SOFT MATTER, 2012, 8 (12) : 3470 - 3477
  • [26] Integrative deign of a poly(ethylene glycol)-poly(propylene glycol)-alginate hydrogel to control three dimensional biomineralization
    Cha, Chaenyung
    Kim, Eun-Seok
    Kim, Il Won
    Kong, Hyunjoon
    BIOMATERIALS, 2011, 32 (11) : 2695 - 2703
  • [27] A poly(ethylene glycol) three-dimensional bone marrow hydrogel
    Jansen, Lauren E. E.
    Kim, Hyuna
    Hall, Christopher L.
    McCarthy, Thomas P.
    Lee, Michael J.
    Peyton, Shelly R.
    BIOMATERIALS, 2022, 280
  • [28] Preparation and Characterization of Silicone Hydrogel Lens Containing Poly(ethylene glycol)
    Jang, Ha Na
    Chung, Youn Bok
    Kim, Sung Soo
    POLYMER-KOREA, 2009, 33 (02) : 169 - 174
  • [29] The Effect of Ultraviolet Light on Biorepulsive Hydrogel Poly(ethylene glycol) Films
    Zhao, Zhiyong
    Yan, Rui
    Zharnikov, Michael
    ACS APPLIED POLYMER MATERIALS, 2021, 3 (07): : 3446 - 3454
  • [30] Improvement of Permeability of Poly(vinyl alcohol) Hydrogel by Using Poly(ethylene glycol) as Porogen
    Zhang, Yi
    Ye, Lin
    POLYMER-PLASTICS TECHNOLOGY AND ENGINEERING, 2011, 50 (08) : 776 - 782
12345