Molecular characteristics of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae causing intra-abdominal infections from 9 tertiary hospitals in China

被引：26

作者：

Liao, Kang ^{[1
]}

Chen, Yili ^{[1
]}

Wang, Menghe ^{[1
]}

Guo, Penghao ^{[1
]}

Yang, Qiwen ^{[2
]}

Ni, Yuxing ^{[3
]}

Yu, Yunsong ^{[4
]}

Hu, Bijie ^{[5
]}

Sun, Ziyong ^{[6
]}

Huang, Wenxiang ^{[7
]}

Wang, Yong ^{[8
]}

Wu, Anhua ^{[9
]}

Feng, Xianju ^{[10
]}

Luo, Yanping ^{[11
]}

Hu, Zhidong ^{[12
]}

Chu, Yunzhuo ^{[13
]}

Chen, Shulan ^{[14
]}

Cao, Bin ^{[15
]}

Su, Jianrong ^{[16
]}

Gui, Bingdong ^{[17
]}

Duan, Qiong ^{[18
]}

Zhang, Shufang ^{[19
]}

Shao, Haifeng ^{[20
]}

Kong, Haishen ^{[21
]}

Xu, Yingchun ^{[2
]}

机构：

[1] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Lab Med, Guangzhou 510080, Guangdong, Peoples R China

[2] Chinese Acad Med Sci, Peking Union Med Coll Hosp, Peking Union Med Coll, Beijing 100730, Peoples R China

[3] Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Shanghai 200025, Peoples R China

[4] Zhejiang Univ, SirRunRun Shaw Hosp, Sch Med, Hangzhou 310016, Zhejiang, Peoples R China

[5] Fu Dan Univ, Zhong Shan Hosp, Shanghai 200032, Peoples R China

[6] Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Wuhan 430030, Peoples R China

[7] Chongqing Med Univ, Affiliated Hosp 1, Chongqing 400016, Peoples R China

[8] Shandong Univ, Shandong Prov Hosp, Jinan 250021, Peoples R China

[9] Cent Southern Univ, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China

[10] Zhengzhou Univ, Affiliated Hosp 1, Zhenzhou 450052, Peoples R China

[11] Gen Hosp Peoples Liberat Army, Beijing 100853, Peoples R China

[12] Tianjin Med Univ, Gen Hosp, Tianjin 300052, Peoples R China

[13] Chinese Med Univ, Affiliated Hosp 1, Shenyang 110001, Peoples R China

[14] Haerbin Med Coll, Affiliated Hosp 1, Haerbin 150081, Peoples R China

[15] Capital Med Coll, Chaoyang Hosp, Beijing 100020, Peoples R China

[16] Capital Med Coll, Friendship Hosp, Beijing 100020, Peoples R China

[17] Nanchang Univ, Affiliated Hosp 2, Nanchang 330006, Peoples R China

[18] Peoples Hosp, Nanjing 130021, Jilin, Peoples R China

[19] Peoples Hosp Haikou City, Haikou 570208, Peoples R China

[20] Gen Hosp Nanjing Mil Command, Nanjing 210002, Jiangsu, Peoples R China

[21] Zhejiang Univ, Affiliated Hosp 1, Hangzhou 310003, Peoples R China

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2017年 / 87卷 / 01期

关键词：

Intra-abdominal infections; Molecular characteristics; ESBL; RESISTANCE TRENDS SMART; CTX-M; EUROPE; ENTEROBACTERIACEAE; UPDATE; CIAO;

D O I：

10.1016/j.diagmicrobio.2016.10.007

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background: Recently, the emergence of multidrug-resistant organisms such as extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae has raised considerable concern regarding the appropriate treatment of intra-abdominal infections (Yds). In this study, we investigated the molecular characteristics of ESBL among clinical isolates of Escherichia coli and Klebsiella pneumoniae causing lAls and their pattern of antimicrobial resistance, which can provide useful information about the epidemiology and risk factors associated with these infections. Materials and methods: One hundred sixty-seven E.co li and 47 K. pneumoniae ESBL-producing strains causing IAIs were collected from 9 hospitals in China, during 2012 and 2013. The antimicrobial susceptibility profile of these strains was determined. Polymerase chain reaction and sequencing were performed to identify genes for beta-lactamase (bIaTEM, b1aSHV, blaOXA-1-like, and blaCTX-M). The isolates were also analyzed by pulsed-field gel electrophoresis (PFGE). Results: In 167 ESBL-producing E. coli strains, 104 strains (623%) were positive for CTX-M, and 9 strains (5.39%) were positive for SHV. Among the 47 K. pneumoniae strains, 35 strains (74.5%) were positive for SHV-2a, 12 strains (25.5%) were positive for CTX-M. No TEM-type and OXA-1-like strain was detected among all the ESBL-producing strains. Regarding the CTX-M-positive E. coli and K. pneumoniae strains, CFX-M-15 was the most common genotype in E. coli and K. pneumoniae strains, accounting for 28.7% and 17.0%, respectively, followed by CTX-M-55 accounting for 16.2% and 2.13%, respectively; the remaining genotypes included CTX-M-123 and CTX-M-82. PFGE showed that E.coli and K. pneumoniae ESBL-producing strains causing lAls were diverse and that emerging resistance may not be due to the dissemination of national clones. Conclusion: The present study revealed that in ESBL-producing strains causing IAIs in China, the most common genotype for E.coli was CTX-M-15 and for K. pneumoniae was SHV-2a. However, there was a wide diversity of strains causing 'Ms among the ESBL-producing E. coll. and K. pneumoniae. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：45 / 48

页数：4

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