Anti-biofilm activity of silver nanoparticles against different microorganisms

被引:178
作者
Martinez-Gutierrez, Fidel [1 ]
Boegli, Laura [2 ]
Agostinho, Alessandra [2 ]
Morales Sanchez, Elpidio [3 ]
Bach, Horacio [4 ]
Ruiz, Facundo [5 ]
James, Garth [2 ]
机构
[1] UASLP, Fac Ciencias Quim, San Luis Potosi, Mexico
[2] Montana State Univ, Ctr Biofilm Engn, Bozeman, MT 59717 USA
[3] Univ Autonoma San Luis Potosi, Dept Fis Matemat, San Luis Potosi, Mexico
[4] Univ British Columbia, Dept Med, Div Infect Dis, Vancouver, BC, Canada
[5] UASLP, Fac Ciencias, San Luis Potosi, Mexico
关键词
silver nanoparticles; biofilm; Pseudomonas aeruginosa; CDC reactor; polycarbonate membrane; anti-biofilm activity; PSEUDOMONAS-AERUGINOSA; ANTIBIOTIC PENETRATION; ESCHERICHIA-COLI; BACTERIAL; RESISTANCE; CYTOTOXICITY; LIMITATION; DIFFUSION; EFFICACY; GROWTH;
D O I
10.1080/08927014.2013.794225
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Biofilms confer protection from adverse environmental conditions and can be reservoirs for pathogenic organisms and sources of disease outbreaks, especially in medical devices. The goal of this research was to evaluate the anti-biofilm activities of silver nanoparticles (AgNPs) against several microorganisms of clinical interest. The antimicrobial activity of AgNPs was tested within biofilms generated under static conditions and also under high fluid shears conditions using a bioreactor. A 4-log reduction in the number of colony-forming units of Pseudomonas aeruginosa was recorded under turbulent fluid conditions in the CDC reactor on exposure to 100mgml(-1) of AgNPs. The antibacterial activity of AgNPs on various microbial strains grown on polycarbonate membranes is reported. In conclusion, AgNPs effectively prevent the formation of biofilms and kill bacteria in established biofilms, which suggests that AgNPs could be used for prevention and treatment of biofilm-related infections. Further research and development are necessary to translate this technology into therapeutic and preventive strategies.
引用
收藏
页码:651 / 660
页数:10
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