Spectroscopic studies on the formation and thermal stability of DNA triplexes with a benzoannulated δ-carboline-oligonucleotide conjugate

被引：23

作者：

Eick, Andrea ^{[1
]}

Xiao, Zhou ^{[1
]}

Langer, Peter ^{[2
]}

Weisz, Klaus ^{[1
]}

机构：

[1] Ernst Moritz Arndt Univ Greifswald, Inst Biochem, D-17487 Greifswald, Germany

[2] Univ Rostock, Inst Chem, D-18059 Rostock, Germany

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2008年 / 16卷 / 20期

关键词：

DNA triplex; delta-carboline; oligonucleotide conjugate; spectroscopy;

D O I：

10.1016/j.bmc.2008.09.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A benzoannulated delta-carboline with a phenyl substituent has been covalently tethered to the 3 '-end of a triplex-forming oligonucleotide and its ability to bind and stabilize DNA triple helices has been examined by various spectroscopic methods. UV thermal melting experiments were conducted with different hairpin duplexes and with a complementary single-stranded oligonucleotide as targets for the conjugate. The delta-carboline ligand preferentially binds triplexes over duplexes and leads to a temperature increase of the triplex-to-duplex transition by up to 23 degrees C. The results obtained from UV, CD and. fluorescence measurements suggest that the delta-carboline ligand exhibits specific interactions with a triplex and favors binding by intercalation at the triplex-duplex junction. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：9106 / 9112

页数：7

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