DNA repair in Etoposide-induced DNA damage in lymphocytes of breast cancer patients and healthy women

被引：0

作者：

Teixeira, Ana Claudia ^{[1
]}

dos Santos, Raquel Alves ^{[1
]}

Poersch, Aline ^{[1
]}

Angotti Carrara, Helio Humberto ^{[2
]}

de Andrade, Jurandyr Moreira ^{[2
]}

Takahashi, Catarina Satie ^{[1
,3
]}

机构：

[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14040900 Ribeirao Preto, SP, Brazil

[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Gynecol & Obstet, BR-14040900 Ribeirao Preto, SP, Brazil

[3] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP, Brazil

来源：

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE | 2009年 / 2卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

Micronucleus Test; Comet Assay; breast cancer; DNA repair; etoposide; CELL GEL-ELECTROPHORESIS; COMET ASSAY; GENETIC INSTABILITY; IN-VITRO; GENOTOXICITY; SUSCEPTIBILITY; POLYMORPHISMS; XRCC1;

D O I：

暂无

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The present study evaluated the basal DNA damage and the cellular response to this damage induced by in vitro administration of Etoposide in lymphocytes donated by twenty untreated breast cancer (BC) patients and twenty age-matched healthy women. Micronucleus (MN) and alkaline Comet assays were performed in cultured peripheral blood lymphocytes (PBL) according to a standard protocol for in vitro treatment with various concentrations of Etoposide or a control. For the Comet Assay, three samples of cells were collected: T-0 (immediately preceding treatment of the cultures), T-1 (immediately after completion of the treatment) and T-2 (four hours after completion of the treatment). MN frequency in the BC group treated with 25 mu M Etoposide (19.1 +/- 7.35) was significantly higher than the control (10.9 +/- 9.87) group. In the alkaline Comet Assay, both the BC group and the healthy women showed the ability to repair Etoposide-induced DNA damage within 4 hours of reincubation.

引用

页码：280 / 288

页数：9

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