Oriented immobilization of antibodies by a self-assembled monolayer of 2-(biotinamido)ethanethiol for immunoarray preparation

被引：18

作者：

Jung, SH

Son, HY

Yuk, JS

Jung, JW

Kim, KH

Lee, CH

Hwang, H

Ha, KS ^{[1
]}

机构：

[1] Kangwon Natl Univ, Coll Med, Dept Mol & Cellular Biochem, Chunchon 200701, Kangwon Do, South Korea

[2] Kangwon Natl Univ, Coll Med, Nano Bio Sensor Res Ctr, Chunchon 200701, Kangwon Do, South Korea

[3] Kangwon Natl Univ, Dept Chem, Chunchon 200701, Kangwon Do, South Korea

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2006年 / 47卷 / 01期

关键词：

2-(biotinamido)ethanethiol; bifunctional linker; immunoarrays; spectral SPR biosensor;

D O I：

10.1016/j.colsurfb.2005.07.007

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Orientation of antibodies is very important in the preparation of immunoarrays to keep the activity of antibodies on solid surfaces. Thus, we synthesized a new bifunctional compound, 2-(biotinamido)ethanethiol, and investigated whether the thiol compound is useful to analyze antibody-antigen interactions on immunoarrays with a spectral SPR biosensor. The synthesized organic thiol was characterized by nuclear magnetic resonance spectroscopy and mass spectrometry. 2-(Biotinamido)ethanethiol formed a monolayer on a gold surface and properly immobilized antibodies via streptavidin and biotinylated protein G. Optimal molar ratio of 2-(biotinamido)ethanethiol and mercaptohexanol for antigen-antibody interactions was 1:2. Thus, 2-(biotinamido)ethanethiol is an useful bifunctional linker in the preparation of immunoarrays on gold surfaces. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：107 / 111

页数：5

共 16 条

[1] Polyelectrolyte adsorption processes characterized in situ using the quartz crystal microbalance technique: alternate adsorption properties in ultrathin polymer films [J].