Mucosal and systemic immunization elicited by Newcastle disease virus (NDV) transgenic plants as antigens

被引：52
作者：
Berinstein, A
Vazquez-Rovere, C
Asurmendi, S
Gómez, E
Zanetti, F
Zabal, O
Tozzini, A
Grand, DC
Taboga, O
Calamante, G
Barrios, H
Hopp, E
Carrillo, E
机构：
[1] INTA, CICVyA, Inst Biotecnol, RA-1712 Castelar, Buenos Aires, Argentina
[2] Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina
[3] INTA, CICVyA, Inst Virol, RA-1712 Castelar, Buenos Aires, Argentina
[4] Univ Nacl Lujan, RA-6700 Lujan, Buenos Aires, Argentina
来源：
VACCINE | 2005年 / 23卷 / 48-49期
关键词：
edible vaccine; transgenic plants; mucosal immunity;
D O I：
10.1016/j.vaccine.2005.06.033
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
As a step towards developing a safe and effective edible vaccine against Newcastle disease virus (NDV), we have explored the use of plants genetically engineered to express viral proteins. We report the construction of transgenic potato plants expressing the genes coding for immunogenic proteins of NDV under the regulation of CaMV 35S promoter and its immunogenicity in mice. All mice receiving transgenic plant extracts in icomplete Freund adjuvant produced specific anti-NDV antibodies. Animals fed with transgenic leaves showed a specific response aganist NDV. Detection of IgA released from in vitro-cultured intestinal tissue fragments indicated the presence of IgA-secreting cells in the gut. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码：5583 / 5589
页数：7
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