A highly sensitive fluorescent probe based on simple pyrazoline for Zn2+ in living neuron cells

被引：53

作者：

Zhang, Zhe ^{[1
]}

Wang, Fang-Wu ^{[2
]}

Wang, Sheng-Qing ^{[1
]}

Ge, Fei ^{[1
]}

Zhao, Bao-Xiang ^{[1
]}

Miao, Jun-Ying ^{[2
]}

机构：

[1] Shandong Univ, Inst Organ Chem, Sch Chem & Chem Engn, Jinan 250100, Peoples R China

[2] Shandong Univ, Sch Life Sci, Inst Dev Biol, Jinan 250100, Peoples R China

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2012年 / 10卷 / 43期

基金：

中国国家自然科学基金;

关键词：

MITOCHONDRIAL ZINC IONS; AQUEOUS-SOLUTION; CHARGE-TRANSFER; SENSOR; CHEMOSENSOR; CYTOTOXICITY; HOMEOSTASIS; MOLECULE; RELEASE;

D O I：

10.1039/c2ob26375k

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We develop a pyrazoline-based fluorescent sensor for biological Zn2+ detection. The sensor shows good binding selectivity for Zn2+ over competing metal with 40-fold fluorescence enhancement in response to Zn2+. The new probe is cell-permeable and can be used to detect intracellular zinc ions in living neuron cells.

引用

页码：8640 / 8644

页数：5

共 42 条

[1] CHELATION-ENHANCED FLUORESCENCE OF ANTHRYLAZAMACROCYCLE CONJUGATE PROBES IN AQUEOUS-SOLUTION [J].

AKKAYA, EU ;

HUSTON, ME ;

CZARNIK, AW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (09) :3590-3593

[2] A highly sensitive two-photon fluorescent probe for mitochondrial zinc ions in living tissue [J].

Baek, Na Young ;

Heo, Cheol Ho ;

Lim, Chang Su ;

Masanta, Goutam ;

Cho, Bong Rae ;

Kim, Hwan Myung .

CHEMICAL COMMUNICATIONS, 2012, 48 (38) :4546-4548

[3] The galvanization of biology: A growing appreciation for the roles of zinc [J].

Berg, JM ;

Shi, YG .

SCIENCE, 1996, 271 (5252) :1081-1085

[4] Understanding Zinc Quantification with Existing and Advanced Ditopic Fluorescent Zinpyr Sensors [J].

Buccella, Daniela ;

Horowitz, Joshua A. ;

Lippard, Stephen J. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 133 (11) :4101-4114

[5]

BUSH AI, 1994, J BIOL CHEM, V269, P12152

[6] RAPID INDUCTION OF ALZHEIMER A-BETA AMYLOID FORMATION BY ZINC [J].

BUSH, AI ;

PETTINGELL, WH ;

MULTHAUP, G ;

PARADIS, MD ;

VONSATTEL, JP ;

GUSELLA, JF ;

BEYREUTHER, K ;

MASTERS, CL ;

TANZI, RE .

SCIENCE, 1994, 265 (5177) :1464-1467

[7] Evidence for a zinc uptake transporter in human prostate cancer cells which is regulated by prolactin and testosterone [J].

Costello, LC ;

Liu, YY ;

Zou, J ;

Franklin, RB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (25) :17499-17504

[8] A Highly Selective Turn-On Colorimetric, Red Fluorescent Sensor for Detecting Mobile Zinc in Living Cells [J].

Du, Pingwu ;

Lippard, Stephen J. .

INORGANIC CHEMISTRY, 2010, 49 (23) :10753-10755

[9] Zinc transporters and the cellular trafficking of zinc [J].

Eide, David J. .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2006, 1763 (07) :711-722

[10] A wavelength and lifetime responsive cryptate-containing fluorescent probe for zinc ions in water [J].

Felton, Cara E. ;

Harding, Lindsay P. ;

Jones, Jennifer E. ;

Kariuki, Benson M. ;

Pope, Simon J. A. ;

Rice, Craig R. .

CHEMICAL COMMUNICATIONS, 2008, (46) :6185-6187

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