Specific interactions between adenosine and streptavidin/avidin

被引：3

作者：

Bing, Tao ^{[1
]}

Chang, Tianjun ^{[1
,2
]}

Qi, Cui ^{[1
,2
]}

Zhang, Nan ^{[1
,2
]}

Liu, Xiangjun ^{[1
]}

Shangguan, Dihua ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2012年 / 22卷 / 23期

关键词：

Molecular interaction; Adenosine; Avidin; Streptavidin; Aptamer; CHIRAL STATIONARY-PHASE; TRYPTOPHAN ACCESSIBILITY; BIOTIN COMPLEX; SERUM ALBUMINS; IN-VITRO; FLUORESCENCE; APTAMERS; AVIDIN; BINDING; MOLECULES;

D O I：

10.1016/j.bmcl.2012.09.088

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The screening of ligands against proteins plays important role in drug discovery and biological research. Using a dye labelled Streptavidin binding aptamer (SBA) as a competitive reporter probe, we found that adenosine bound to streptavidin specifically. Fluorescence spectral analysis showed that adenosine bound to both avidin and streptavidin with the K-ds in the range of 0.1-0.2 mM, and these bindings can be blocked by biotin. Although streptavidin and avidin are well-known and widely used in bioanalysis, their biological role is still a riddle so far. Since adenosine is a ubiquitous physiological regulator present in cells, our finding provides new clues for the understanding of the functions of both proteins. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：7052 / 7055

页数：4

共 41 条

[1] Conservative secondary structure motif of streptavidin-binding aptamers generated by different laboratories [J].

Bing, Tao ;

Yang, Xiaojuan ;

Mei, Hongcheng ;

Cao, Zehui ;

Shangguan, Dihua .

BIOORGANIC & MEDICINAL CHEMISTRY, 2010, 18 (05) :1798-1805

[2] Aptamers come of age - at last [J].

Bunka, David H. J. ;

Stockley, Peter G. .

NATURE REVIEWS MICROBIOLOGY, 2006, 4 (08) :588-596

[3] SITE-DIRECTED MUTAGENESIS STUDIES OF THE HIGH-AFFINITY STREPTAVIDIN-BIOTIN COMPLEX - CONTRIBUTIONS OF TRYPTOPHAN RESIDUE-79, RESIDUE-108, AND RESIDUE-120 [J].

CHILKOTI, A ;

TAN, PH ;

STAYTON, PS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (05) :1754-1758

[4]

DIAMANDIS EP, 1991, CLIN CHEM, V37, P625

[5] FLUORESCENCE QUENCHING STUDIES WITH PROTEINS [J].

EFTINK, MR ;

GHIRON, CA .

ANALYTICAL BIOCHEMISTRY, 1981, 114 (02) :199-227

[6] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].

ELLINGTON, AD ;

SZOSTAK, JW .

NATURE, 1990, 346 (6287) :818-822

[7] Small-molecule screening made simple for a difficult target with a signaling nucleic acid aptamer that reports on deaminase activity [J].

Elowe, Nadine H. ;

Nutiu, Razvan ;

Allah-Hassani, Abdellah ;

Cechetto, Jonathan D. ;

Hughes, Donald W. ;

Li, Yingfu ;

Brown, Eric A. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (34) :5648-5652

[8] Interaction of bovine (BSA) and human (HSA) serum albumins with ionic surfactants: spectroscopy and modelling [J].

Gelamo, EL ;

Silva, CHTP ;

Imasato, H ;

Tabak, M .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2002, 1594 (01) :84-99

[9] STUDIES ON THE BIOTIN-BINDING SITES OF AVIDIN AND STREPTAVIDIN - TYROSINE RESIDUES ARE INVOLVED IN THE BINDING-SITE [J].

GITLIN, G ;

BAYER, EA ;

WILCHEK, M .

BIOCHEMICAL JOURNAL, 1990, 269 (02) :527-530

[10]

Gonzalez M, 1997, J BIOL CHEM, V272, P11288

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