Purification of Bioactive Lipopeptides Produced by Bacillus subtilis Strain BIA

被引：45

作者：

Alajlani, Muaaz ^{[1
]}

Shiekh, Abid ^{[2
]}

Hasnain, Shahida ^{[2
,3
]}

Brantner, Adelheid ^{[1
]}

机构：

[1] Graz Univ, Dept Pharmacognosy, Inst Pharmaceut Sci, Univ Pl 4-1, A-8010 Graz, Austria

[2] Univ Punjab, Fac Life Sci, Dept Microbiol & Mol Genet, Lahore, Pakistan

[3] Women Univ Multan, Dept Microbiol & Mol Genet, Fac Life Sci, Multan, Pakistan

来源：

CHROMATOGRAPHIA | 2016年 / 79卷 / 21-22期

关键词：

Purification; Lipopeptides; TLC; Gel-filtration; RP-HPLC; MALDI-TOF-MS; ITURIN-A; MASS-SPECTROMETRY; SURFACTIN; BIOSURFACTANTS; ANTIBIOTICS; COPRODUCER; CELLS;

D O I：

10.1007/s10337-016-3164-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacillus subtilis strain BIA was used for the production of bioactive lipopeptides. Different extraction and purification methods were assayed as liquid-liquid extraction, and acid and ammonium sulfate precipitation followed by TLC, SPE, and gel filtration. Active fractions were further purified using RP-HPLC. The molecular mass of the purified product from HPLC was determined through Tris-Tricine SDS-PAGE and MALDI-TOF-MS. The results revealed that Bacillus subtilis strain BIA produced surfactin and iturin like compounds. Coproduction of surfactin and iturin like compounds by this strain is a remarkable trait for a potential biocontrol agent. This paper also includeds techniques that have been developed for the optimal and convenient extraction of bioactive lipopeptides from microbial origin.

引用

页码：1527 / 1532

页数：6

共 34 条

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