Purification of Bioactive Lipopeptides Produced by Bacillus subtilis Strain BIA

被引：45

作者：

Alajlani, Muaaz ^{[1
]}

Shiekh, Abid ^{[2
]}

Hasnain, Shahida ^{[2
,3
]}

Brantner, Adelheid ^{[1
]}

机构：

[1] Graz Univ, Dept Pharmacognosy, Inst Pharmaceut Sci, Univ Pl 4-1, A-8010 Graz, Austria

[2] Univ Punjab, Fac Life Sci, Dept Microbiol & Mol Genet, Lahore, Pakistan

[3] Women Univ Multan, Dept Microbiol & Mol Genet, Fac Life Sci, Multan, Pakistan

来源：

CHROMATOGRAPHIA | 2016年 / 79卷 / 21-22期

关键词：

Purification; Lipopeptides; TLC; Gel-filtration; RP-HPLC; MALDI-TOF-MS; ITURIN-A; MASS-SPECTROMETRY; SURFACTIN; BIOSURFACTANTS; ANTIBIOTICS; COPRODUCER; CELLS;

D O I：

10.1007/s10337-016-3164-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacillus subtilis strain BIA was used for the production of bioactive lipopeptides. Different extraction and purification methods were assayed as liquid-liquid extraction, and acid and ammonium sulfate precipitation followed by TLC, SPE, and gel filtration. Active fractions were further purified using RP-HPLC. The molecular mass of the purified product from HPLC was determined through Tris-Tricine SDS-PAGE and MALDI-TOF-MS. The results revealed that Bacillus subtilis strain BIA produced surfactin and iturin like compounds. Coproduction of surfactin and iturin like compounds by this strain is a remarkable trait for a potential biocontrol agent. This paper also includeds techniques that have been developed for the optimal and convenient extraction of bioactive lipopeptides from microbial origin.

引用

页码：1527 / 1532

页数：6

共 34 条

[1] Surfactin and iturin A effects on Bacillus subtilis surface hydrophobicity
Ahimou, F
Jacques, P
Deleu, M
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 2000, 27 (10) : 749 - 754
[2] Simple and rapid isolation of a novel antibiotic from Bacillus subtilis Mz-7
Al-Ajlani, MM
Hasnain, S
[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2006, 29 (05) : 639 - 647
[3] [Anonymous], 1994, METHODS GEN MOL BACT
[4] DIRECT DETECTION OF AN ANTIMICROBIAL PEPTIDE OF PEDIOCOCCUS-ACIDILACTICI IN SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS
BHUNIA, AK
JOHNSON, MC
RAY, B
[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1987, 2 (05): : 319 - 322
[5] Brueckner H, 1984, J CHROMATOGR, V19, P188
[6] Predictive, structure-based model of amino acid recognition by nonribosomal peptide synthetase adenylation domains
Challis, GL
Ravel, J
Townsend, CA
[J]. CHEMISTRY & BIOLOGY, 2000, 7 (03): : 211 - 224
[7] Lipopeptides from Bacillus and Paenibacillus spp.: A Gold Mine of Antibiotic Candidates
Cochrane, Stephen A.
Vederas, John C.
[J]. MEDICINAL RESEARCH REVIEWS, 2016, 36 (01) : 4 - 31
[8] ENHANCED PRODUCTION OF SURFACTIN FROM BACILLUS-SUBTILIS BY CONTINUOUS PRODUCT REMOVAL AND METAL CATION ADDITIONS
COOPER, DG
MACDONALD, CR
DUFF, SJB
KOSARIC, N
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1981, 42 (03) : 408 - 412
[9] Interfacial and emulsifying properties of lipopeptides from Bacillus subtilis
Deleu, M
Razafindralambo, H
Popineau, Y
Jacques, P
Thonart, P
Paquot, M
[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 1999, 152 (1-2) : 3 - 10
[10] BIOSURFACTANTS AND AQUEOUS 2-PHASE FERMENTATION
DROUIN, CM
COOPER, DG
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1992, 40 (01) : 86 - 90

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