Characterization and Comparison of Methacrylic Acid with 2-Acrylamido-2-methyl-1-propanesulfonic Acid in the Preparation of Monolithic Column for Capillary Electrochromatography

被引：0
作者：
Horiguchi, Daisuke [1 ]
Ohyama, Kaname [1 ,2 ]
Masunaga, Tomoko [1 ]
Fujita, Yoshiko [1 ]
Ali, Marwa Fathy Bakr [1 ]
Kishikawa, Naoya [1 ]
Kuroda, Naotaka [1 ]
机构：
[1] Nagasaki Univ, Grad Sch Biomed Sci, Dept Environm & Pharmaceut Sci, Nagasaki 852, Japan
[2] Nagasaki Univ, Res Ctr Genom Instabil & Carcinogenesis NRGIC, Nagasaki 852, Japan
来源：
JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2013年 / 51卷 / 05期
关键词：
RIGID POLYMER MONOLITHS; POROUS PROPERTIES; SEPARATION MEDIA; PHASE; MICROEXTRACTION; CHROMATOGRAPHY; PERFORMANCE; EFFICIENCY; CHEMISTRY;
D O I：
10.1093/chromsci/bms158
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Butyl methacrylate (BMA)-ethylene dimethacrylate (EDMA)-methacrylic acid (MAA) and BMA-EDMA-2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) monolithic columns were prepared by varying the percentage of ionic monomers for capillary electrochro-matography. Monolithic columns with a higher content of ionic monomers provided better column efficiency, and the performance of BMA-EDMA-MAA monoliths was better than BMA-EDMA-AMPS. To characterize and optimize BMA-EDMA-MAA monoliths, the effects of the content of cross-linker and the total monomer in the polymerization mixture on column performance were also studied. Plate heights of 8.2 mu m for the unretained solute (thiourea) and 12.6 mu m for the retained solute (naphthalene) were achieved with a monolithic column using 2.5% MAA (Column I).
引用
收藏
页码：425 / 429
页数：5
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