Enhanced wound-healing capability with inherent antimicrobial activities of usnic acid incorporated poly(?-caprolactone)/decellularized extracellular matrix nanofibrous scaffold

被引：22

作者：

Chandika, Pathum ^{[1
,2
,3
]}

Khan, Fazlurrahman ^{[3
,4
]}

Heo, Seong-Yong ^{[5
]}

Kim, Young-Mog ^{[3
,4
,6
]}

Yi, Myunggi ^{[1
,2
,4
]}

Jung, Won-Kyo ^{[1
,2
,3
,4
]}

机构：

[1] Pukyong Natl Univ, Div Smart Healthcare, Major Biomed Engn, Pusan 48513, South Korea

[2] Pukyong Natl Univ, New Sr Healthcare Innovat Ctr BK21 Plus, Pusan 48513, South Korea

[3] Pukyong Natl Univ, Natl Key Res Inst Univ, Marine Integrated Biomed Technol Ctr, Pusan 48513, South Korea

[4] Pukyong Natl Univ, Res Ctr Marine Integrated Technol B, Pusan 48513, South Korea

[5] Korea Inst Ocean Sci & Technol, Jeju Marine Res Ctr, Jeju 63349, South Korea

[6] Pukyong Natl Univ, Major Food Sci & Technol, Pusan 48513, South Korea

来源：

BIOMATERIALS ADVANCES | 2022年 / 140卷

基金：

新加坡国家研究基金会;

关键词：

Decellularized extracellular matrix; PCL; Usnic acid; Antimicrobial; Anti-biofilm; Electrospune nanofiber; Full -thickness wound healing; IMPLANTABLE DEVICE; TISSUE; FABRICATION; HYDROGEL; PROGRESS; BIOFILM; RELEASE;

D O I：

10.1016/j.bioadv.2022.213046

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

An extracellular matrix-mimicking, biodegradable tissue-engineered skin substitute with improved antibacterial, antibiofilm, and wound healing capabilities is essential in skin tissue regeneration applications. The purpose of this study was to develop a novel biodegradable composite nanofibrous poly(epsilon-caprolactone) (PCL)/decellular-ized extracellular matrix (dECM) scaffolds loaded with usnic acid (UA); (PEU), where UA is employed as an antibacterial agent as well as a wound-healing accelerator. The architecture and fiber structure of the scaffolds were examined using scanning electron microscopy, and the results revealed that the average diameters decreased as the dECM content increased. The chemical composition, changes in the crystalline structure, ho-mogeneity, and thermal stability of the nanofiber scaffolds with different material compositions were determined using Fourier-transform infrared spectroscopy, X-ray diffraction, differential scanning calorimetry, and ther-mogravimetric analysis, respectively. The composite nanofibrous scaffolds exhibited strong antibacterial activity against various bacterial species, such as Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, and Cutibactrium acnes, and fungal pathogens (such as Candida albicans). Additionally, the composite nanofibrous scaffolds exhibited biofilm inhibition properties against Klebsiella pneumoniae and Pseudomonas aeruginosa. An evaluation of the appearance of in vivo full-thickness excisional wounds treated with the composite nanofiber scaffolds, as well as a histological analysis of the wounds 21 days after surgery, revealed that treatment with nanofibrous PEU scaffolds enhanced wound healing. This study reveals that the proposed composite nanofibrous PEU scaffold has substantial potential for treating infectious full-thickness wounds.

引用

页数：15

共 57 条

[1] Biomimetic PCL-gelatin based nanofibers loaded with ciprofloxacin hydrochloride and quercetin: A potential antibacterial and anti-oxidant dressing material for accelerated healing of a full thickness wound [J].