In situ fluorescent profiling of living cell membrane proteins at a single-molecule level

被引：28
|
作者：
Fan, Yuanyuan [1 ]
Li, Lu [1 ]
Lu, Meng [1 ]
Si, Haibin [1 ]
Tang, Bo [1 ]
机构：
[1] Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes,Inst Mol & Nano Sci, Minist Educ,Collaborat Innovat Ctr Functionalized, Jinan 250014, Shandong, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 28期
基金：
中国博士后科学基金; 中国国家自然科学基金;
关键词：
APTAMER; BINDING; PROBES;
D O I：
10.1039/c9cc00244h
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We propose a signal amplification method that enables visualization analysis of membrane proteins on living cells at a single-molecule level. Using the proposed method, we achieved imaging of PTK7 membrane proteins on HeLa cells and analyzed the down-regulated expression of EpCAM on the MCF-7 cell surface during epithelial-mesenchymal transition (EMT).
引用
收藏
页码：4043 / 4046
页数：4
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