Overexpression of brain-derived neurotrophic factor in the hippocampus protects against post-stroke depression

被引:52
作者
Chen, Hao-hao [1 ]
Zhang, Ning [1 ]
Li, Wei-yun [2 ]
Fang, Ma-rong [2 ]
Zhang, Hui [3 ]
Fang, Yuan-shu [3 ]
Ding, Ming-xing [1 ]
Fu, Xiao-yan [1 ]
机构
[1] Jinhua Polytech, Sch Med, Med Mol Biol Lab, Jinhua, Zhejiang, Peoples R China
[2] Zhejiang Univ, Sch Med, Inst Neurosci, Hangzhou 310003, Zhejiang, Peoples R China
[3] Jinhua Food & Drug Adm, Ctr Expt Anim, Jinhua, Zhejiang, Peoples R China
关键词
nerve regeneration; brain injury; brain-derived neurotrophic factor; lentivirus; post-stroke depression; depression-like behavior; hippocampus; cerebral ischemia; sucrose solution consumption; open field test; chronic unpredictable mild stress; western blot assay; neural regeneration; FACTOR EXPRESSION; RATS; BDNF; NEUROGENESIS; ANTIDEPRESSANTS; HYPOTHESIS; STRESS; INTEGRATION; DISORDERS; BEHAVIOR;
D O I
10.4103/1673-5374.165510
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Post-stroke depression is associated with reduced expression of brain-derived neurotrophic factor (BDNF). In this study, we evaluated whether BDNF overexpression affects depression-like behavior in a rat model of post-stroke depression. The middle cerebral artery was occluded to produce a model of focal cerebral ischemia. These rats were then subjected to isolation-housing combined with chronic unpredictable mild stress to generate a model of post-stroke depression. A BDNF gene lentiviral vector was injected into the hippocampus. At 7 days after injection, western blot assay and real-time quantitative PCR revealed that BDNF expression in the hippocampus was increased in depressive rats injected with BDNF lentivirus compared with depressive rats injected with control vector. Furthermore, sucrose solution consumption was higher, and horizontal and vertical movement scores were increased in the open field test in these rats as well. These findings suggest that BDNF overexpression in the hippocampus of post-stroke depressive rats alleviates depression-like behaviors.
引用
收藏
页码:1427 / 1432
页数:6
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