Whole-Genome SNP Sequencing of Selected Progeny Individuals Allows Anchoring of Draft Genome Sequence to a Linkage Map for the Apple Scab Pathogen Venturia inaequalis

被引：1

作者：

Rees, D. J. G. ^{[1
]}

Celton, J. -M. ^{[1
]}

Cristoffels, A.

Sargent, D. J.

Xu, X.

机构：

[1] Univ Western Cape, Dept Biotechnol, Private Bag X17, ZA-7535 Bellville, South Africa

来源：

INTERNATIONAL SYMPOSIUM ON MOLECULAR MARKERS IN HORTICULTURE | 2010年 / 859卷

关键词：

Venturia inaequalis; sequencing; SNP; bin mapping;

D O I：

10.17660/ActaHortic.2010.859.34

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Determining the position and order of contigs within an organism's genome remains a technical challenge in a majority of sequencing projects. To exploit contemporary technologies for DNA sequencing, we developed a strategy for whole genome SNP sequencing allowing the positioning of contigs onto a linkage map using the bin mapping method. We demonstrated the utility of this strategy by accurately determining the joint genotypes of a portion of large contigs and validated our method by mapping SSR markers developed from bin-mapped sequences from a draft genome assembly of the apple scab pathogen, Venturia inaequalis.

引用

页码：295 / 300

页数：6

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