Isolation and Characterization of Thermostable Amylase Producing Bacteria from Hot Water Springs of Gir National Forest

被引：0

作者：

Kapadia, C. V. ^{[1
]}

Gandhi, B. ^{[1
]}

Patel, N. ^{[1
]}

Kansara, R. ^{[1
]}

Vaidya, H. ^{[1
]}

机构：

[1] Navsari Agr Univ, Dept Biotechnol, Navsari 396450, India

来源：

JOURNAL OF PURE AND APPLIED MICROBIOLOGY | 2012年 / 6卷 / 03期

关键词：

Thermostable alpha- amylase; Bacillus thermodenitrificans str.T4; BACILLUS; LIPASE;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The present study is aimed at assessing the ability of the microbial community of hot water spring to produce different industrial important enzymes. Identification was based on biochemical, morphological measures and response of different growth parameters.15 potential isolates of different thermostable enzyme producer were obtained during primary screening. Secondary screening of these bacterial isolates yielded a high thermostable alpha- amylase producing isolate. Among all the 6 isolates, isolate no. 2 gave remarkable response to various growth parameters. The optimum temperature and initial medium pH for amylase synthesis by the organism were 70 degrees C and 7.0 respectively. Phylogenetic analysis on the basis of 16S rRNA gene sequence of isolate no. 2 revealed that it was new and next closest homologue was found to be Bacillus thermodenitrificans str.T4

引用

页码：1235 / 1241

页数：7

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