Development of Real-Time Polymerase Chain Reaction for Detection of Borrelia burgdorferi sensu lato in China

被引：5

作者：

Fu, Yuguang ^{[1
]}

Liu, Zhijie ^{[1
]}

Guan, Guiquan ^{[1
]}

Niu, Qingli ^{[1
]}

Li, Youquan ^{[1
]}

Yang, Jifei ^{[1
]}

Ren, Qiaoyun ^{[1
]}

Ma, Miling ^{[1
]}

Liu, Aihong ^{[1
]}

Peng, Yulv ^{[1
]}

Luo, Jianxun ^{[1
]}

Yin, Hong ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Key Lab Vet Parasitol Gansu Prov, Key Lab Grazing Anim Dis MOA,State Key Lab Vet Et, Lanzhou 730046, Peoples R China

来源：

VECTOR-BORNE AND ZOONOTIC DISEASES | 2012年 / 12卷 / 05期

关键词：

Borrelia burgdorferi sensu lato; Real-time PCR; Yak and sheep; LYME-DISEASE SPIROCHETE; TICKS; REGION; AGENT;

D O I：

10.1089/vbz.2011.0692

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Universal primers and probes were selected on the basis of the 16S rRNA gene sequence of Borrelia burgdorferi in GenBank (R), and a real-time polymerase chain reaction (PCR) method for detection of B. burgdorferi was established. The results showed that this method could specifically detect the B31 strain (Borrelia burgdorferi sensu stricto), the BO23 strain (Borrelia afzelii), and the SZ strain (Borrelia garinii), without cross-reaction with genome DNA of Theileria (T. luwenshuni, T. uilenbergi, T. sinensis, T. annulata, T. sergenti, T. annulata), Babesia (B. bigemina, B. ovate, B. sp. (Xinjiang)), Anaplasma (A. marginale, A. ovis), Mycoplasma mycoides subsp. capri, and Chlamydia psittaci, which are the infective pathogens to yak and/or sheep. The sensitivity of this real-time PCR is 10(4) times greater than that of a conventional PCR. The real-time PCR was able to amplify 16S rRNA gene from as few as 22.88 fg genomic DNA of B. burgdorferi sensu lato. Tick DNAs from 369 field samples collected from Shangzhi City of Heilongjiang Province were tested, resulting in an infection rate of 42.80%, and a total of 332 genomic DNAs from the blood of 186 yaks and 146 sheep in the Gannan Tibetan Autonomous Prefecture of Gansu Province were tested, resulting in 24.19% positive rate for the yaks and 39.04% positive rate for the sheep.

引用

页码：341 / 345

页数：5

共 21 条

[1] Risk factors associated with ixodid tick species distributions in the Basque region in Spain [J].