Molecular cloning of Kazal-type proteinase inhibitor of the shrimp Fenneropenaeus chinensis

被引：32

作者：

Kong, Hee Jeong ^{[1
]}

Cho, Hyun Kook ^{[1
]}

Park, Eun-Mi ^{[1
]}

Hong, Gyeong-Eun ^{[1
]}

Kim, Young-Ok ^{[1
]}

Nam, Bo-Hye ^{[1
]}

Kim, Woo-Jin ^{[1
]}

Lee, Sang-Jun ^{[1
]}

Han, Hyon Sob ^{[1
]}

Jang, In-Kwon ^{[2
]}

Lee, Chang Hoon ^{[3
]}

Cheong, JaeHun ^{[4
]}

Choi, Tae-Jin ^{[1
,5
]}

机构：

[1] Natl Fisheries Res & Dev Inst, Biotechnol Res Inst, Pusan 619705, South Korea

[2] Natl Fisheries Res & Dev Inst, W Sea Mariculture Res Inst, Chungnam 357945, South Korea

[3] Pusan Natl Univ, Res Grp Marine & Silver Biotechnol, Pusan 609735, South Korea

[4] Pusan Natl Univ, Dept Mol Biol, Pusan 609735, South Korea

[5] Pukyong Natl Univ, Dept Microbiol, Pusan 608737, South Korea

来源：

FISH & SHELLFISH IMMUNOLOGY | 2009年 / 26卷 / 01期

关键词：

Kazal type proteinase inhibitor; Shrimp; Fenneropenaeus chinensis; Hemocyte; cDNA; INNATE IMMUNITY; EXPRESSION; WSSV;

D O I：

10.1016/j.fsi.2008.03.023

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Proteinase inhibitors play important roles in host defence systems involving blood coagulation and pathogen digestion. We isolated and characterized a cDNA clone for a Kazal-type proteinase inhibitor (KPI) from a hemocyte cDNA library of the oriental white shrimp Fenneropenaeus chinensis. The KPI gene consists of three exons and two introns. KPI cDNA contains an open reading frame of 396 bp, a polyadenylation signal sequence AATAAA, and a poly (A) tail. KPI cDNA encodes a polypeptide of 131 amino acids with a putative signal peptide of 21 amino acids. The deduced amino acid sequence of KPI contains two homologous Kazal domains, each with six conserved cysteine residues. The mRNA of KPI is expressed in the hemocytes of healthy shrimp, and the higher expression of KPI transcript is observed in shrimp infected with the white spot syndrome virus (WSSV), suggesting a potential rote for KPI in host defence mechanisms. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：109 / 114

页数：6

共 30 条

[1] Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea [J].

Asahida, T ;

Kobayashi, T ;

Saitoh, K ;

Nakayama, I .

FISHERIES SCIENCE, 1996, 62 (05) :727-730

[2] NATURAL PROTEIN PROTEINASE-INHIBITORS AND THEIR INTERACTION WITH PROTEINASES [J].

BODE, W ;

HUBER, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 204 (02) :433-451

[3]

BREATHNACH R, 1981, ANNU REV BIOCHEM, V50, P349, DOI 10.1146/annurev.bi.50.070181.002025

[4] PROTEIN PROTEASE INHIBITORS IN INSECTS AND COMPARISON WITH MAMMALIAN INHIBITORS [J].

EGUCHI, M .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1993, 105 (3-4) :449-456

[5]

FRIEDRICH T, 1993, J BIOL CHEM, V268, P16216

[6]

Iwanaga S, 2005, J BIOCHEM MOL BIOL, V38, P128

[7] Recombinant expression and characterization of five-domain Kazal-type serine proteinase inhibitor of black tiger shrimp (Penaeus monodon) [J].

Jarasrassamee, B ;

Supungul, P ;

Panyim, S ;

Klinbunga, S ;

Rimphanichayakit, V ;

Tassanakajon, A .

MARINE BIOTECHNOLOGY, 2005, 7 (01) :46-52

[8] Multiple sequence alignment with Clustal x [J].

Jeanmougin, F ;

Thompson, JD ;

Gouy, M ;

Higgins, DG ;

Gibson, TJ .

TRENDS IN BIOCHEMICAL SCIENCES, 1998, 23 (10) :403-405

[9] A four-Kazal domain protein in Litopenaeus vannamei hemocytes [J].

Jiménez-Vega, F ;

Vargas-Albores, F .

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2005, 29 (05) :385-391

[10] PURIFICATION AND CDNA CLONING OF A 4-DOMAIN KAZAL PROTEINASE-INHIBITOR FROM CRAYFISH BLOOD-CELLS [J].

JOHANSSON, MW ;

KEYSER, P ;

SODERHALL, K .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 223 (02) :389-394

← 1 2 3 →