Development of simple sequence repeat markers for the soybean rust fungus, Phakopsora pachyrhizi

被引:11
作者
Anderson, Sharon J. [1 ]
Stone, Christine L. [1 ]
Posada-Buitrago, Martha Lucia [2 ,3 ]
Boore, Jeffrey L. [2 ,3 ]
Neelam, Beena A. [4 ]
Stephens, Robert M. [4 ]
Luster, Douglas G. [1 ]
Frederick, Reid D. [1 ]
Pedley, Kerry F. [1 ]
机构
[1] USDA ARS, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA
[2] Joint Genome Inst, Walnut Creek, CA 94598 USA
[3] Lawrence Berkeley Natl Lab, US Dept Energy, Walnut Creek, CA USA
[4] NCI Frederick, SAIC Frederick Inc, Adv Biomed Comp Ctr, Adv Technol Program, Ft Detrick, MD 21702 USA
关键词
basidiomycetes; microsatellites; Phakopsora pachyrhizi; soybean rust; SSR;
D O I
10.1111/j.1755-0998.2008.02272.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Twenty-four simple sequence repeat markers were developed for Phakopsora pachyrhizi, a fungal pathogen of soybean (Glycine max) and other legumes. All 24 of the loci were evaluated on 28 isolates of P. pachyrhizi. Twenty-one loci were polymorphic, with allelic diversity ranging from two to eight alleles, and null alleles were observed for eight of the 24 loci. A preliminary screen with the closely related species, P. meibomiae, indicated that these primer pairs are specific to P. pachyrhizi.
引用
收藏
页码:1310 / 1312
页数:3
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