Novel Caspase-Suicide Proteins for Tamoxifen-Inducible Apoptosis

被引：14

作者：

Chu, Yuanyuan ^{[1
]}

Senghaas, Niklas ^{[1
]}

Koester, Reinhard W. ^{[1
]}

Wurst, Wolfgang ^{[1
,2
,3
]}

Kuehn, Ralf ^{[1
,3
]}

机构：

[1] Helmholtz Zentrum Munchen, Inst Dev Genet, German Res Ctr Environm Hlth, D-85764 Munich, Germany

[2] Max Planck Inst Psychiat, Dept Mol Neurogenet, D-80804 Munich, Germany

[3] Tech Univ Munich, Dept Dev Genet, D-8000 Munich, Germany

来源：

GENESIS | 2008年 / 46卷 / 10期

关键词：

caspase; apoptosis; tamoxifen; cell ablation; estrogen receptor;

D O I：

10.1002/dvg.20426

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Taking advantage of a mutant estrogen receptor ligand binding domain (ER T), we developed novel Caspase fusion proteins for inducible apoptosis. We show that Caspase-ERT2 fusion proteins become specifically activated by the synthetic ligand 4-OH-tamoxifen and rapidly induce apoptotic cell death in human, murine, and zebrafish cells. This novel tool for targeted cell ablation greatly facilitates the generation of disease models as well as developmental and regeneration studies in model organisms. genesis 46:530-536, 2008. (C) 2008 Wiley-Liss, Inc.

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收藏

页码：530 / 536

页数：7

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