Analysis of receptor-ligand binding by photoaffinity cross-linking

被引：5
作者：
Xu Bin [1 ]
Wu Ling
机构：
[1] Virginia Tech, Dept Biochem, Blacksburg, VA 24061 USA
来源：
SCIENCE CHINA-CHEMISTRY | 2014年 / 57卷 / 02期
关键词：
photoaffinity cross-linking; receptor-ligand interaction; photoscanning; bifunctional photo-probe; biosynthetic photo-probe; novel receptor discovery; DIABETES-ASSOCIATED MUTATIONS; PROTEIN-PROTEIN INTERACTIONS; SITE-SPECIFIC INCORPORATION; NONCANONICAL AMINO-ACIDS; INSULIN-RECEPTOR; ESCHERICHIA-COLI; GENETIC-CODE; MAMMALIAN-CELLS; INTEGRIN ALPHA(V)BETA(3); COUPLED-RECEPTORS;
D O I：
10.1007/s11426-013-5045-z
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Photoaffinity cross-linking is a fast developing technology for biomolecular interactions, including receptor-ligand binding. The chemical mechanisms of the most commonly used photoactivatable probes and their respective photochemistry are summarized. This review focuses on the expanding utilities of this technology as a result of recent advances in the (i) identification of receptor contact sites, (ii) monitoring ligand-induced receptor conformational changes, (iii) identification of global binding surfaces, (iv) binding mode analysis using bifunctional photo-probes, (v) application of biosynthetic photo-probes, and (vi) examples of novel target discovery using this technology. Limitations and future potential of this approach are also discussed.
引用
收藏
页码：232 / 242
页数：11
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