Detection of ivermectin resistance by a larval development test-Back to the past or a step forward?

被引：23

作者：

Dolinska, M. ^{[1
]}

Koenigova, A. ^{[1
]}

Letkova, V. ^{[2
]}

Molnar, L. ^{[3
]}

Varady, M. ^{[1
]}

机构：

[1] Slovak Acad Sci, Inst Parasitol, Kosice 04001, Slovakia

[2] Univ Vet Med & Pharm, Dept Epizootol & Parasitol, Kosice 04181, Slovakia

[3] Univ Vet Med & Pharm, Clin Birds & Exot Anim, Kosice 04181, Slovakia

来源：

VETERINARY PARASITOLOGY | 2013年 / 198卷 / 1-2期

关键词：

Anthelmintic resistance; In vitro detection; Haemonchus contortus; Ivermectin; COUNT REDUCTION TEST; IN-VITRO METHODS; HAEMONCHUS-CONTORTUS; ANTHELMINTIC RESISTANCE; OSTERTAGIA-CIRCUMCINCTA; LEVAMISOLE RESISTANCE; VETERINARY IMPORTANCE; MACROCYCLIC LACTONES; DEVELOPMENT ASSAY; SOUTH-AFRICA;

D O I：

10.1016/j.vetpar.2013.07.043

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The aim of this study was to evaluate the potential of the larval development test for the detection of ivermectin (IVM) resistance in Haemonchus contortus of sheep. Single infections with 5000 third-stage larvae of five resistant and two susceptible isolates of H. contortus were given to sheep. Fecal samples were collected four times during patency, and the micro-agar version of the larval development test (MALDT) was performed. Three macrocyclic lactone drugs (IVM, eprinomectin and IVM aglycone) were tested. The results of the tests are presented as LC50 and LC99 values. The MALDT was well able to distinguish between susceptible and resistant isolates. Resistance factors (RF) for the LC99 values were generally higher than those obtained by comparing LC50 values. The highly resistant isolates were readily distinguishable from the susceptible isolates, particularly when using IVM aglycone and eprinomectin, with RFs above 20. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：154 / 158

页数：5

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