Genomic DNA isolation from dried blood using gelatin-coated magnetic particles

被引：0

作者：

Intorasoot, Sorasak ^{[1
]}

Techateerawat, Jintara ^{[1
]}

Intorasoot, Amornrat ^{[2
]}

机构：

[1] Chiang Mai Univ, Div Clin Microbiol, Dept Med Technol, Fac Associated Med Sci, Chiang Mai 50200, Thailand

[2] Chiang Mai Univ, Dept Microbiol, Fac Med, Chiang Mai 50200, Thailand

来源：

CURRENT SCIENCE | 2013年 / 105卷 / 01期

关键词：

Dried blood; DNA extraction; gelatin; magnetic particles; PLASMID DNA; PURIFICATION; THALASSEMIA;

D O I：

暂无

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Gelatin-coated magnetic particles were implemented for isolation of human genomic DNA from dried blood spotted onto glass microfibre filters. The DNA quality and quantity were compared to a silica-based membrane purification kit. Conditions for dried blood storage were varied for DNA stability determination. Approximately three times higher DNA yield was recovered by coated magnetic particles than the silica-based kit. In addition, the quality of extracted DNA was determined using beta-actin gene amplification by polymerase chain reaction (PCR). It was implied that genomic DNA was stable for at least 28 days in a dried condition. Gelatin-coated magnetic particles are rapid, simple and useful for DNA extraction in field applications.

引用

页码：81 / 84

页数：4

共 19 条

[1] Hepatitis C virus RNA in dried serum spotted onto filter paper is stable at room temperature [J].