DEFECTIVE KERNEL1 (DEK1) Regulates Cell Walls in the Leaf Epidermis

被引:24
作者
Amanda, Dhika [1 ]
Doblin, Monika S. [1 ]
Galletti, Roberta [2 ]
Bacic, Antony [1 ]
Ingram, Gwyneth C. [2 ]
Johnson, Kim L. [1 ]
机构
[1] Univ Melbourne, Australian Res Council Ctr Excellence Plant Cell, Sch Biosci, Parkville, Vic 3010, Australia
[2] Univ Lyon, Ecole Normale Super Lyon, CNRS,Unite Mixte Rech 0879, INRA,Lab Reprod & Dev Plantes,Unite Mixte Rech 56, F-69342 Lyon, France
基金
澳大利亚研究理事会;
关键词
LIPID TRANSFER PROTEINS; PECTIN METHYLESTERASE; ARABIDOPSIS-THALIANA; FUNCTIONAL-ANALYSIS; IMAGE-ANALYSIS; GROWTH-CONTROL; PLANT-GROWTH; BIOSYNTHESIS; CALPAIN; DIFFERENTIATION;
D O I
10.1104/pp.16.01401
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The plant epidermis is crucial to survival, regulating interactions with the environment and controlling plant growth. The phytocalpain DEFECTIVE KERNEL1 (DEK1) is a master regulator of epidermal differentiation and maintenance, acting upstream of epidermis-specific transcription factors, and is required for correct cell adhesion. It is currently unclear how changes in DEK1 lead to cellular defects in the epidermis and the pathways through which DEK1 acts. We have combined growth kinematic studies, cell wall analysis, and transcriptional analysis of genes downstream of DEK1 to determine the cause of phenotypic changes observed in DEK1-modulated lines of Arabidopsis (Arabidopsis thaliana). We reveal a novel role for DEK1 in the regulation of leaf epidermal cell wall structure. Lines with altered DEK1 activity have epidermis-specific changes in the thickness and polysaccharide composition of cell walls that likely underlie the loss of adhesion between epidermal cells in plants with reduced levels of DEK1 and changes in leaf shape and size in plants constitutively overexpressing the active CALPAIN domain of DEK1. Calpain-overexpressing plants also have increased levels of cellulose and pectins in epidermal cell walls, and this is correlated with the expression of several cell wall-related genes, linking transcriptional regulation downstream of DEK1 with cellular effects. These findings significantly advance our understanding of the role of the epidermal cell walls in growth regulation and establish a new role for DEK1 in pathways regulating epidermal cell wall deposition and remodeling.
引用
收藏
页码:2204 / 2218
页数:15
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