Analytical evaluation of the Capillarys 2 Flex piercing for routine haemoglobinopathies diagnosis

被引：11

作者：

Agouti, I. ^{[1
,2
]}

Merono, F. ^{[3
]}

Bonello-Palot, N. ^{[1
,3
]}

Badens, C. ^{[1
,3
,4
]}

机构：

[1] Hop Enfants La Timone, AP HM, Lab Genet Mol, F-13385 Marseille 5, France

[2] Hop Enfants La Timone, Ctr Reference Malad Rares Thalassemie, F-13385 Marseille 5, France

[3] Aix Marseille Univ, Fac Med, UMR S 910, Marseille, France

[4] Aix Marseille Univ, Fac Pharm, Lab Chim Analyt, Marseille, France

来源：

INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY | 2013年 / 35卷 / 02期

关键词：

Capillary electrophoresis; haemoglobin; haemoglobinopathies; ELECTROPHORESIS METHOD; ANALYZER;

D O I：

10.1111/ijlh.12000

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

To evaluate the analytical performance of a new capillary electrophoresis instrument, the Capillarys 2 Flex piercing (Sebia, France), allowing the separation and quantitative estimation of the different haemoglobin fractions from whole blood, in capped primary tube.The analytical precision for the determination of HbA2 and HbF percentages was satisfactory and within the range of previously published results for HPLC methods. The correlation between Capillarys 2 Flex Piercing and Bio-Rad Variant II HPLC system showed a linear correlation for HbA2, HbF and HbS measurements, and the analysis interpretation was the same whatever the method used. Conversely to HPLC method, the capillary's electrophoresis technology allowed HbE and Hb Lepore fraction separation from HbA2. We showed that the Capillarys 2 Flex Piercing is suitable for haemoglobinopathies diagnosis and screening and offers an excellent alternative to HPLC techniques as a first-line method or for confirmatory analysis.

引用

页码：217 / 221

页数：5

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