Physiological and transcriptomic analyses reveal a response mechanism to cold stress in Santalum album L. leaves

被引：58

作者：

Zhang, Xinhua ^{[1
]}

Teixeira da Silva, Jaime A. ^{[2
]}

Niu, Meiyun ^{[1
]}

Li, Mingzhi ^{[3
]}

He, Chunmei ^{[1
]}

Zhao, Jinhui ^{[1
]}

Zeng, Songjun ^{[1
]}

Duan, Jun ^{[1
]}

Ma, Guohua ^{[1
]}

机构：

[1] Chinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou, Guangdong, Peoples R China

[2] POB 7,Miki Cho PO,Ikenobe 3011-2, Miki, Kagawa 7610799, Japan

[3] Genepioneer Biotechnol Co Ltd, Nanjing 210014, Jiangsu, Peoples R China

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

中国国家自然科学基金;

关键词：

RNA-SEQ DATA; LOW-TEMPERATURE; FUNCTIONAL-CHARACTERIZATION; SUPEROXIDE-DISMUTASE; SIGNAL-TRANSDUCTION; FREEZING TOLERANCE; GENE-EXPRESSION; CBF REGULON; SANDALWOOD; PATHWAY;

D O I：

10.1038/srep42165

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Santalum album L. (Indian sandalwood) is an economically important plant species because of its ability to produce highly valued perfume oils. Little is known about the mechanisms by which S. album adapts to low temperatures. In this study, we obtained 100,445,724 raw reads by paired-end sequencing from S. album leaves. Physiological and transcriptomic changes in sandalwood seedlings exposed to 4 degrees C for 0-48 h were characterized. Cold stress induced the accumulation of malondialdehyde, proline and soluble carbohydrates, and increased the levels of antioxidants. A total of 4,424 differentially expressed genes were responsive to cold, including 3,075 cold-induced and 1,349 cold-repressed genes. When cold stress was prolonged, there was an increase in the expression of cold-responsive genes coding for transporters, responses to stimuli and stress, regulation of defense response, as well as genes related to signal transduction of all phytohormones. Candidate genes in the terpenoid biosynthetic pathway were identified, eight of which were significantly involved in the cold stress response. Gene expression analyses using qRT-PCR showed a peak in the accumulation of SaCBF2 to 4, 50-fold more than control leaves and roots following 12 h and 24 h of cold stress, respectively. The CBF-dependent pathway may play a crucial role in increasing cold tolerance.

引用

页数：18

共 75 条

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