Efficient incorporation of unnatural amino acids into proteins in Escherichia coli

被引：243
|
作者：
Ryu, YH
Schultz, PG
机构：
[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
来源：
NATURE METHODS | 2006年 / 3卷 / 04期
关键词：
D O I：
10.1038/NMETH864
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a single-plasmid system for the efficient bacterial, expression of mutant proteins containing unnatural amino acids at specific sites designated by amber nonsense codons. In this system, multiple copies of a gene encoding an amber suppressor tRNA derived from a Methanocaldococcus jannaschii tyrosyl-tRNA (MjtRNA(CUA)(Tyr)) are expressed under control of the proK promoter and terminator, and a gene encoding the desired mutant M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS) is expressed under control of a mutant glnS (glnS') promoter.
引用
收藏
页码：263 / 265
页数：3
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