Anther Culture Response in Wheat (Triticum aestivum L.) Genotypes with HMW Alleles

被引：4

作者：

Barakat, M. N. ^{[1
]}

Al-Doss, A. A. ^{[1
,2
]}

Elshafei, A. A. ^{[1
]}

Moustafa, K. A. ^{[2
]}

Ahmed, E. I. ^{[2
]}

机构：

[1] King Saud Univ, Ctr Excellence Biotechnol Res, Plant Genet Manipulat & Genom Breeding Grp, Riyadh, Saudi Arabia

[2] King Saud Univ, Plant Prod Dept, Coll Food Sci & Agr, Riyadh, Saudi Arabia

来源：

CEREAL RESEARCH COMMUNICATIONS | 2012年 / 40卷 / 04期

关键词：

anther culture; double haploids; haploids; wheat; DOUBLED-HAPLOID PRODUCTION; COLCHICINE; CALLUS;

D O I：

10.1556/CRC.40.2012.0011

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The objective of this study was to develop a simple anther culture protocol for a range of Saudi wheat genotypes. Seven wheat genotypes were evaluated in anther culture on five different medium protocols for their ability to initiate callus and green plants. The estimates of significance for the effects of genotypes, and medium protocols used, and their interactions on callus induction, callus weight and shoot formation derived from anther explants indicated that the in vitro traits were significantly influenced by the genotypes, medium protocols, and their interactions. The percentage of explants that developed calli ranged from 0.41% (Lang) to 15.39% (Irena) averaged across the five medium protocols with an average 4.45%. The genotype Irena produced the highest average means of shoot formation (69.65%) across medium protocols. The genotype Yecora Rojo (13.73%) was significantly inferior to all other tested genotypes for shoot formation.

引用

页码：583 / 591

页数：9

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