Lutibacter litoralis gen. nov., sp nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment

A rod-shaped marine bacterium, designated strain CL-TF09(T), isolated from a tidal flat in Ganghwa, Korea, was characterized based on its physiological and biochemical features, fatty acid profile and phylogenetic position. 16S rRNA gene sequence analysis revealed a clear affiliation with the family Flavobacteriaceae. Strain CL-TF09(T) showed the closest phylogenetic relationship with the genera Tenacibaculum and Polaribacter; sequence similarities between CL-TF09(T) and the type strains of Tenacibaculum and Polaribacter species ranged from 90.7 to 91.8%. Cells of strain CL-TF09(T) were non-motile and grew on solid media as yellow colonies. The strain grew in the presence of 1-5% sea salts, within a temperature range of 5-30 degrees C and at pH 7-8. The strain had iso-C-15:0 3-OH (17.4%), iso-C-15:0 (16.7%), anteiso-C-15:0 (15.1%) and iso-C-16:0 3-OH (13.4%) as predominant fatty acids. The DNA G + C content was 33.9 mol%. Based on the physiological, fatty acid composition and phylogenetic data presented, strain CL-TF09(T) is considered to represent a novel genus and species of the family Flavobacteriaceae, for which the name Lutibacter litoralis gen. nov., sp. nov. is proposed. The type strain is CL-TF09(T) (= KCCM 42118(T)=JCM 13034(T)).