ARF1, a transcription factor that binds to auxin response elements

被引:762
作者
Ulmasov, T [1 ]
Hagen, G [1 ]
Guilfoyle, TJ [1 ]
机构
[1] UNIV MISSOURI,DEPT BIOCHEM,COLUMBIA,MO 65211
关键词
D O I
10.1126/science.276.5320.1865
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The plant hormone auxin regulates plant physiology by modulating the interaction of transcription factors with auxin response elements (AuxREs) of the affected genes. A transcription factor, Auxin Response Factor 1 (ARF1), that binds to the sequence TGTCTC in AuxREs was cloned from Arabidopsis by using a yeast one-hybrid system. ARF1 has an amino-terminal DNA-binding domain related to the carboxyl terminus of the maize transactivator Viviparous-1. Sequence requirements for ARF1 binding in vitro are identical to those that confer auxin responsiveness in vivo. The carboxyl terminus of ARF1 contains two motifs found in the Aux/IAA class of proteins and appears to mediate protein-protein interactions.
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页码:1865 / 1868
页数:4
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