Structural determination and antitumor activities of a water-soluble polysaccharide from Mortierella hepiali

被引：7

作者：

Wang, Ying ^{[1
,2
]}

Zhao, Huan ^{[2
]}

Miao, Xiaoliang ^{[2
]}

Liu, Dan ^{[2
]}

Jiang, Huixing ^{[3
]}

Liu, Peipei ^{[2
]}

Wang, Yufeng ^{[4
]}

Yin, Hongping ^{[1
,2
]}

机构：

[1] China Pharmaceut Univ, State Key Lab Nat Med, Nanjing 210009, Jiangsu, Peoples R China

[2] China Pharmaceut Univ, Sch Life Sci & Technol, Dept Microbiol & Biochem, Nanjing 210009, Jiangsu, Peoples R China

[3] Nanjing Univ Tradit Chinese Med, Nanjing Tradit Chinese Med Hosp, Affiliated Hosp 3, Nanjing 210010, Jiangsu, Peoples R China

[4] Jiangsu Yurun Meat Ind Grp Co Ltd, Nanjing 210041, Jiangsu, Peoples R China

来源：

FITOTERAPIA | 2013年 / 86卷

关键词：

Mortierella hepiali; Structure characterization; Cytotoxicity; Antitumor activities; CULTURED CORDYCEPS-SINENSIS; IMMUNOMODULATORY ACTIVITY; HYPOGLYCEMIC ACTIVITY; ANTIOXIDANT ACTIVITY; FRUITING BODIES; MYCELIA; TUMOR; MICE; MACROPHAGES; MILITARIS;

D O I：

10.1016/j.fitote.2013.01.001

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A water-soluble polysaccharide (MHP-1) isolated from cultured Mortierella hepiali was obtained by hot-water extraction, DEAE-cellulose 52 anion-exchange, and Sephadex G-25 gel permeation chromatography. Its structural characteristics were investigated by PMP pre-column derivation, partial hydrolysis with acid, methylation analysis, as well as FTIR and NMR spectroscopy. Data revealed that MHP-1 possessed a backbone consisting of the repeating unit -> 2)-beta-D-Manp-(1, 6)-beta-D-Glcp-(1 -> and 3)-beta-D-Manp-(1 ->, branching at O-4 of -> 2)-beta-D-Manp-(1, and terminated with beta-D-Manp. The antitumor effects of MHP-1 on H-22 tumor-bearing mice were studied. Results showed that MHP-1 significantly inhibited the growth of implanted H-22 tumors in mice in vivo, but not on H-22, A549, and Hep-G2 cells in vitro. Serum and spleen cells were separated to evaluate the concentrations of TNF-alpha by ELISA kit. The release of TNF-alpha was markedly induced by MHP-1 in a dose-dependent manner. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：13 / 18

页数：6

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