Objective: The goal of this study was to assess the quality of life and negative emotions of patients with cervical cancer after neoadjuvant chemotherapy in early psychological nursing. Methods: A total of 154 patients with cervical cancer who received neoadjuvant chemotherapy in Linyi People's Hospital were included and divided into two groups, with 77 cases in each group. The quality of life, negative emotions, mental health, and sleep quality were compared between the two groups after different nursing methods. Results: There was no significant difference in the general data, quality of life, negative emotions, mental health, and sleep quality scores before nursing intervention (P>0.05). The total effective rate after neoadjuvant adjuvant chemotherapy (NACT) treatment was 71.42% in the observation group and 68.83% in the control group. There was no significant difference in efficacy between the two groups (P>0.05). After the nursing intervention, quality of life scores, sleep quality, mental health, and anxiety and depression of the two groups were significantly improved compared with those before nursing intervention (all P<0.05). After the nursing intervention, the scores of life quality, anxiety and depression, mental health and sleep quality of the observation group were significantly improved and were better than those of the control group (all P<0.05), especially in terms of the scores of emotional function (P<0.001) and somatic pain (P=0.012). Conclusion: Psychological nursing intervention can improve the quality of life, reduce negative emotions such as anxiety and depression, improve mental health, and improve sleep quality of patients with cervical cancer. Thus, psychological nursing intervention is worthy of clinical application in the management of cancer.