Reverse-Transcription PCR (RT-PCR)

被引：82

作者：

Bachman, Julia ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA

来源：

LABORATORY METHODS IN ENZYMOLOGY: RNA | 2013年 / 530卷

关键词：

D O I：

10.1016/B978-0-12-420037-1.00002-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and over developmental time courses. This serves as a form of expression profiling, but typically as a candidate approach. RT-PCR is also commonly used to clone cDNAs for further use with other molecular biology techniques (e.g., see Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates and Circularized RT-PCR (cRT-PCR): analysis of RNA 5' ends, 3' ends, and poly(A) tails).

引用

页码：67 / 74

页数：8